Preparation and immunogenicity of an inactivated hepatitis A vaccine

Pellegrini, Vittoria, Fineschi, Nicoletta, Matteucci, G., Marsili, I., Nencioni, L., Puddu, M., Garelick, Hemda ORCID logoORCID: and Zuckerman, Arie J. (1993) Preparation and immunogenicity of an inactivated hepatitis A vaccine. Vaccine, 11 (3) . pp. 383-387. ISSN 0264-410X [Article] (doi:10.1016/0264-410X(93)90204-B)


A hepatitis A vaccine was prepared by formaldehyde inactivation of purified hepatitis A virus (HAV) LSH/S strain grown on human diploid MRC-5 cells. The vaccine was devoid of residual infectivity in vitro and failed to induce in marmoset monkeys any pathological features or variations of haematological and clinical chemistry values. Infectious HAV particles were not detected in faeces and sera of the vaccinated primates by ELISA or after passages in MRC-5 cells. The immunogenicity of the vaccine was evaluated by injecting guinea-pigs with 0.8, 0.2 or 0.05 μg of HAV antigen adsorbed onto 0.5 and 1 mg of Al(OH)3 or 0.3 mg of AlPO4. The antibody response, measured by a competitive radioimmunoassay, was dose- and adjuvant-dependent. One injection of 0.2 μg of AlPO4-adsorbed HAV antigen induced seroconversion in 100% of animals and high levels of specific and neutralizing serum antibodies. A further increase of antibody titres was observed after the second and third inoculations. These results show that this vaccine formulation is safe and immunogenic in animal models, and suggest that it should be evaluated further by human clinical studies.

Item Type: Article
Research Areas: A. > School of Science and Technology > Natural Sciences
ISI Impact: 21
Item ID: 3731
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Depositing User: Devika Mohan
Date Deposited: 18 Jan 2010 07:24
Last Modified: 06 Jun 2019 09:13

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