Hepatitis C antibody profile and viraemia prevalence in adults with severe haemophilia

Tedder, Richard S., Briggs, M., Ring, Christopher J. ORCID: https://orcid.org/0000-0002-9278-0942, Tuke, Philip W., Jones, P., Savidge, G. F., Rodgers, B. and Garson, Jeremy A. (1991) Hepatitis C antibody profile and viraemia prevalence in adults with severe haemophilia. British Journal of Haematology, 79 (3) . pp. 512-515. ISSN 0007-1048 [Article] (doi:10.1111/j.1365-2141.1991.tb08064.x)

Abstract

Sera from 21 patients who had received large amounts of unheated factor VIII concentrate were tested for antibodies to the hepatitis C virus (HCV) by both commercial (Ortho C100) and 'in house' ELISAs. 'In house' assays utilized recombinant structural (core) or non-structural (replicase) HCV proteins generated by a baculovirus expression system. Antibodies to HCV were detected in 100% of the sera by the core protein based ELISA but in only 62% and 19% by the C100 and replicase based ELISAs, respectively. Hepatitis C viraemia was demonstrated in 90% of the patients by in vitro amplification of the 5' non-coding region of the HCV genome. Amplification with primer sets from two other regions of the genome proved less efficient at detecting viraemia. We conclude that the prevalence of hepatitis C infection in haemophiliacs may have been underestimated previously and that almost all HCV-infected patients have evidence of on-going viral replication.

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