Detection of extended-spectrum β-lactamase and carbapenemase activity in Gram-negative bacilli using liquid chromatography - tandem mass spectrometry

Serafim, Vlad, Shah, Ajit J. ORCID: https://orcid.org/0000-0002-2350-6384, Licker, Monica ORCID: https://orcid.org/0000-0002-9245-5883, Horhat, Florin George ORCID: https://orcid.org/0000-0001-6133-0204, Vulpie, Silvana, Musuroi, Corina and Muntean, Delia ORCID: https://orcid.org/0000-0001-9100-4530 (2020) Detection of extended-spectrum β-lactamase and carbapenemase activity in Gram-negative bacilli using liquid chromatography - tandem mass spectrometry. Infection and Drug Resistance, 13 . pp. 4021-4029. ISSN 1178-6973 [Article] (doi:10.2147/IDR.S267160)

Preview	PDF - Published version (with publisher's formatting) Download (2MB) | Preview
	PDF - Final accepted version (with author's formatting) Restricted to Repository staff and depositor only Available under License Creative Commons Attribution-NonCommercial 4.0. Download (744kB)
	Image (JPEG) - Final accepted version (with author's formatting) Restricted to Repository staff and depositor only Available under License Creative Commons Attribution-NonCommercial 4.0. Download (259kB)
	Image (JPEG) - Final accepted version (with author's formatting) Restricted to Repository staff and depositor only Available under License Creative Commons Attribution-NonCommercial 4.0. Download (48kB)

Abstract

PURPOSE: Several mass spectrometry-based methods for antimicrobial sensitivity testing have been described in recent years. They offer an alternative to commercially available testing systems which were considered to have disadvantages in terms of cost- and time-efficiency. The aim of this study was to develop a LC-MS/MS-based antibiotic hydrolysis assay for evaluating antimicrobial resistance of Gram-negative bacteria.

METHODS: Four species of Gram-negative bacilli (Klebsiella pneumoniae, Escherichia coli, Providencia stuartii and Acinetobacter baumannii) were tested against six antibiotics from three different classes: ampicillin, meropenem, imipenem, ceftazidime, ceftriaxone and cefepime. Bacterial suspensions from each species were incubated with a mixture of the six antibiotics. Any remaining antibiotic following incubation were measured using LC-MS/MS. The results were interpreted using measurements obtained for an E. coli strain sensitive to all antibiotics and expressed as percentage of hydrolyzed antibiotic. These were subsequently compared to commercially-available system for the bacteria identification and susceptibility testing.

RESULTS: Overall, LC-MS/MS assay and commercial antimicrobial susceptibility platform results showed good agreement in terms of an organism being resistant/sensitive to an antibiotic. The time required to complete the LC-MS/MS-based hydrolysis test was under 5 h, significantly shorter that commercially available susceptibility testing platforms.

CONCLUSION: By using a sensitive strain for results interpretation and simultaneous use of multiple antibiotics, the proposed protocol offers improved robustness and multiplexing over previously described methods for antibiotic sensitivity testing. Nevertheless, further research is needed before routine assimilation of the method, especially for strains with intermediate resistance.

Actions (login required)

View Item

Statistics

DownloadsShow export options

Export as CSVXMLJSON

Activity Overview

6 month trend

83Downloads

6 month trend

99Hits

Additional statistics are available via IRStats2.