A global multinational survey of cefotaxime-resistant coliforms in urban wastewater treatment plants

Marano, Roberto B M, Fernandes, Telma, Manaia, Célia M, Nunes, Olga, Morrison, Donald, Berendonk, Thomas U, Kreuzinger, Norbert, Telson, Tanel, Corno, Gianluca, Fatta-Kassinos, Despo, Merlin, Christophe, Topp, Edward, Jurkevitch, Edouard, Henn, Leonie, Scott, Andrew, Heß, Stefanie, Slipko, Katarzyna, Laht, Mailis, Kisand, Veljo, Di Cesare, Andrea, Karaolia, Popi, Michael, Stella G, Petre, Alice L, Rosal, Roberto, Pruden, Amy, Riquelme, Virginia, Agüera, Ana, Esteban, Belen, Luczkiewicz, Aneta, Kalinowska, Agnieszka, Leonard, Anne, Gaze, William H, Adegoke, Anthony A, Stenstrom, Thor A, Pollice, Alfieri, Salerno, Carlo, Schwermer, Carsten U, Krzeminski, Pawel, Guilloteau, Hélène, Donner, Erica, Drigo, Barbara, Libralato, Giovanni, Guida, Marco, Bürgmann, Helmut, Beck, Karin, Garelick, Hemda ORCID: https://orcid.org/0000-0003-4568-2300, Tacão, Marta, Henriques, Isabel, Martínez-Alcalá, Isabel, Guillén-Navarro, Jose M, Popowska, Magdalena, Piotrowska, Marta, Quintela-Baluja, Marcos, Bunce, Joshua T, Polo-López, Maria I, Nahim-Granados, Samira, Pons, Marie-Noëlle, Milakovic, Milena, Udikovic-Kolic, Nikolina, Ory, Jérôme, Ousmane, Traore, Caballero, Pilar, Oliver, Antoni, Rodriguez-Mozaz, Sara, Balcazar, Jose L, Jäger, Thomas, Schwartz, Thomas, Yang, Ying, Zou, Shichun, Lee, Yunho, Yoon, Younggun, Herzog, Bastian, Mayrhofer, Heidrun, Prakash, Om, Nimonkar, Yogesh, Heath, Ester, Baraniak, Anna, Abreu-Silva, Joana, Choudhury, Manika, Pantoja Munoz, Leonardo ORCID: https://orcid.org/0000-0001-8364-7595, Krizanovic, Stela, Brunetti, Gianluca, Maile-Moskowitz, Ayella, Brown, Connor and Cytryn, Eddie (2020) A global multinational survey of cefotaxime-resistant coliforms in urban wastewater treatment plants. Environment International, 144 , 106035. pp. 1-11. ISSN 0160-4120 [Article] (doi:10.1016/j.envint.2020.106035)

Preview

PDF - Published version (with publisher's formatting) Available under License Creative Commons Attribution-NonCommercial-NoDerivatives 4.0. Download (1MB) | Preview

Abstract

The World Health Organization Global Action Plan recommends integrated surveillance programs as crucial strategies for monitoring antibiotic resistance. Although several national surveillance programs are in place for clinical and veterinary settings, no such schemes exist for monitoring antibiotic-resistant bacteria in the environment. In this transnational study, we developed, validated, and tested a low-cost surveillance and easy to implement approach to evaluate antibiotic resistance in wastewater treatment plants (WWTPs) by targeting cefotaxime-resistant (CTX-R) coliforms as indicators. The rationale for this approach was: i) coliform quantification methods are internationally accepted as indicators of fecal contamination in recreational waters and are therefore routinely applied in analytical labs; ii) CTX-R coliforms are clinically relevant, associated with extended-spectrum β-lactamases (ESBLs), and are rare in pristine environments. We analyzed 57 WWTPs in 22 countries across Europe, Asia, Africa, Australia, and North America. CTX-R coliforms were ubiquitous in raw sewage and their relative abundance varied significantly (<0.1% to 38.3%), being positively correlated (p < 0.001) with regional atmospheric temperatures. Although most WWTPs removed large proportions of CTX-R coliforms, loads over 10 colony-forming units per mL were occasionally observed in final effluents. We demonstrate that CTX-R coliform monitoring is a feasible and affordable approach to assess wastewater antibiotic resistance status. [Abstract copyright: Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.]

Actions (login required)

View Item

Statistics

DownloadsShow export options

Export as CSVXMLJSON

Activity Overview

6 month trend

54Downloads

6 month trend

84Hits

Additional statistics are available via IRStats2.