Exploitation of nitric oxide donors to control bacterial adhesion on ready‐to‐eat vegetables and dispersal of pathogenic biofilm from polypropylene

Islam, Mohammad, Durie, Ian, Ramadan, Reham, Purchase, Diane ORCID: https://orcid.org/0000-0001-8071-4385 and Marvasi, Massimiliano ORCID: https://orcid.org/0000-0001-8525-7232 (2020) Exploitation of nitric oxide donors to control bacterial adhesion on ready‐to‐eat vegetables and dispersal of pathogenic biofilm from polypropylene. Journal of the Science of Food and Agriculture, 100 (7) . pp. 3078-3086. ISSN 0022-5142 [Article] (doi:10.1002/jsfa.10340)

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Abstract

Background
Nitric oxide (NO) donors have been used to control biofilm formation. NO can be delivered in situ using organic carriers and act as a signaling molecule. Cells exposed to NO shift from biofilm to the planktonic state and are better exposed to the action of disinfectants. In this study, we investigate the capability of the NO donors molsidomine, MAHAMA NONOate, NO‐aspirin and diethylamine NONOate to act as anti‐adhesion agents on ready‐to‐eat vegetables, as well as dispersants to a number of pathogenic biofilms on plastic.

Results
Our results showed that 10pM molsidomine reduced the attachment of Salmonella enterica sv Typhimurium 14 028 to pea shoots and coriander leaves of about 0.5 Log(CFU/leaf) when compared with untreated control. The association of 10 pM molsidomine with 0.006% H2O2 showed a synergistic effect, obtaining a significant reduction in cell collection on the surface of the vegetable of about 1 Log(CFU/leaf). Similar results were obtained for MAHMA NONOate.

We also showed that the association of diethylamine NONOate at 10 mM and 10pM with the quaternary ammonium compound diquat bromide improves the effectiveness of biofilm dispersal by 50% when compared with the donor alone.

Conclusions
Our findings reveal the dual role of NO compounds in biofilm control. Molsidomine, MAHMA NONOate and diethylamine NONOate are good candidates in either preventing biofilm formation or dispersing biofilm, especially when used in conjunction with disinfectants. NO compounds have the potential to be developed into tool‐kit for pro‐active practices for GAPs, HACCP and Cleaning‐in‐place (CIP) protocols in industrial settings where washing is routinely applied.

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Item Type: Article
Research Areas: A. > School of Science and Technology > Natural Sciences
Item ID: 29152
Notes on copyright: This is the peer reviewed version of the following article: Islam, M., Durie, I., Ramadan, R., Purchase, D. and Marvasi, M. (2020), Exploitation of nitric oxide donors to control bacterial adhesion on ready‐to‐eat vegetables and dispersal of pathogenic biofilm from polypropylene. J Sci Food Agric, 100: 3078-3086. doi:10.1002/jsfa.10340, which has been published in final form at https://doi.org/10.1002/jsfa.10340. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
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Depositing User: Diane Purchase
Date Deposited: 24 Feb 2020 09:21
Last Modified: 14 Apr 2020 16:51
URI: https://eprints.mdx.ac.uk/id/eprint/29152

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