Fat removal with hydrolyzed corn starch for real-time qPCR detection of Salmonella enterica in ground beef in 4.5 hours without enrichment

Cossu, Andrea ORCID: https://orcid.org/0000-0003-4086-8640, Witkowsky, Ruth D. and Levin, Robert E. (2014) Fat removal with hydrolyzed corn starch for real-time qPCR detection of Salmonella enterica in ground beef in 4.5 hours without enrichment. Food Control, 46 . pp. 475-479. ISSN 0956-7135 [Article] (doi:10.1016/j.foodcont.2014.06.009)

Abstract

The rapid detection of low number of Salmonella in ground beef ideally requires an effective and economic molecular assay. The molecular analysis for the detection of Salmonella in ground beef by the polymerase chain reaction requires efficient methodology for extraction of targeted cells and effective removal of PCR inhibitors from the sample. The efficacy of hydrolyzed corn starch for the removal of fat along with the use of activated charcoal coated with milk proteins to remove PCR inhibitors were assessed. Salmonella enterica ser. Enteritidis was detected by real-time quantitative PCR at a level of 1 CFU/g of ground beef in 25 g samples containing 7%, 15% or 27% fat without enrichment. This study documents that partially hydrolyzed corn starch functions as effectively as beta-cyclodextrin for selective removal of fat.

Item Type: Article
Research Areas: A. > School of Science and Technology > Natural Sciences
Item ID: 27325
Useful Links:
Depositing User: Andrea Cossu
Date Deposited: 27 Nov 2019 15:20
Last Modified: 27 Nov 2019 15:20
URI: https://eprints.mdx.ac.uk/id/eprint/27325

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