Progesterone represses interleukin-8 and cyclo-oxygenase-2 in human lower segment fibroblast cells and amnion epithelial cells
Loudon, Jenifer A.Z., Elliott, Catherine L., Hills, Frank ORCID: https://orcid.org/0000-0001-8235-7545 and Bennett, Phillip R.
(2003)
Progesterone represses interleukin-8 and cyclo-oxygenase-2 in human lower segment fibroblast cells and amnion epithelial cells.
Biology of Reproduction, 69
(1)
.
pp. 331-337.
ISSN 0006-3363
[Article]
Abstract
Labor is preceded by cervical ripening through upregulation of interleukin (IL)-1β, IL-8, and increased prostaglandin synthesis via inducible type 2 cyclooxygenase (COX-2). Progesterone maintains myometrial quiescence during pregnancy. In this study, we examined the effects of IL-1β and progesterone on IL-8 and prostaglandin E2 (PGE2) synthesis and IL-8 and COX-2 mRNA and promoter activity in amnion cells and lower segment fibroblast (LSF) cells. In both cell types, progesterone had no effect on basal IL-8 or PGE2 synthesis. In LSF cells, IL-1β significantly increased IL-8 and PGE2 synthesis and COX-2 and IL-8 mRNA expression, but progesterone significantly attenuated these effects. In prelabor amnion cells, IL-1β also increased IL-8 and PGE2 synthesis and both COX-2 and IL-8 mRNA and promoter expression; however, progesterone significantly attenuated these effects on IL-8 and PGE2 synthesis and COX-2 expression. In postlabor amnion cells, IL-1β increased IL-8 and PGE2 synthesis and COX-2 expression, but progesterone did not attenuate the effect of IL-1β upon IL-8 synthesis. Progesterone repression of IL-8 and COX-2 in LSF cells suggests that IL-8 and COX-2 have similar regulatory mechanisms in LSF cells and that progesterone may play a role in maintenance of cervical competence. The lack of effect of progesterone on IL-8 in postlabor cells may be the result of downregulation of the progesterone receptor during labor.
Item Type: | Article |
---|---|
Additional Information: | PubMed PMID: 12672669. |
Research Areas: | A. > School of Science and Technology > Natural Sciences > Biomarkers for Cancer group A. > School of Science and Technology > Natural Sciences > Reproductive Biology group A. > School of Health and Education > Centre for Investigative and Diagnostic Oncology A. > School of Science and Technology > Natural Sciences |
ISI Impact: | 35 |
Item ID: | 2501 |
Useful Links: | |
Depositing User: | Repository team |
Date Deposited: | 17 Jun 2009 13:20 |
Last Modified: | 13 Oct 2016 14:14 |
URI: | https://eprints.mdx.ac.uk/id/eprint/2501 |
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