Competition of As and other Group 15 elements for surface binding sites of an extremophilic Acidomyces acidophilus isolated from a historical tin mining site

Chan, Wai Kit, Wildeboer, Dirk ORCID: https://orcid.org/0000-0003-1298-7511, Garelick, Hemda ORCID: https://orcid.org/0000-0003-4568-2300 and Purchase, Diane ORCID: https://orcid.org/0000-0001-8071-4385 (2018) Competition of As and other Group 15 elements for surface binding sites of an extremophilic Acidomyces acidophilus isolated from a historical tin mining site. Extremophiles, 22 (5) . pp. 795-809. ISSN 1431-0651 [Article] (doi:10.1007/s00792-018-1039-2)

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Abstract

An arsenic-resistant fungal strain, designated WKC-1, was isolated from a waste roaster pile in a historical tin mine in Cornwall, UK and successfully identified to be Acidomyces acidophilus using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight tandem mass spectrometry (MALDI-TOF/TOF MS) proteomic-based biotyping approach. WKC-1 showed considerable resistance to As5+ and Sb5+ where the minimal inhibitory concentration (MIC) were 22500 mg L-1 and 100 mg L-1 respectively on Czapex-Dox Agar (CDA) medium; it was substantially more resistant to As5+ than the reference strains CBS 335.97 and CCF 4251. In a modified CDA medium containing 0.02 mg L-1 phosphate, WKC-1 was able to remove 70.30 % of As5+ (100 mg L-1). Sorption experiment showed that the maximum capacity of As5+ uptake was 170.82 mg g-1 dry biomass as predicted by the Langmuir model. The presence of Sb5+ reduced the As5+ uptake by nearly 40%. Based on the Fourier-transform infrared spectroscopy (FT-IR) analysis, we propose that Sb is competing with As for these sorption sites: OH, NH, CH, SO3 and PO4 on the fungal cell surface. To our knowledge, this is the first report on the impact of other Group 15 elements on the biosorption of As5+ in Acidomyces acidophilus.

Item Type: Article
Research Areas: A. > School of Science and Technology > Natural Sciences
Item ID: 24935
Notes on copyright: This is a post-peer-review, pre-copyedit version of an article published in Extremophiles. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00792-018-1039-2
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Depositing User: Diane Purchase
Date Deposited: 12 Sep 2018 17:32
Last Modified: 10 Jun 2021 06:25
URI: https://eprints.mdx.ac.uk/id/eprint/24935

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