High-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples

Hows, Mark E. P., Lacroix, Laurent, Heidbreder, Christian A., Organ, Andrew J. and Shah, Ajit J. ORCID: https://orcid.org/0000-0002-2350-6384 (2004) High-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples. Journal of Neuroscience Methods, 138 (1-2) . pp. 123-132. ISSN 0165-0270 [Article] (doi:10.1016/j.jneumeth.2004.03.021)

Abstract

A rapid liquid chromatography/tandem mass spectrometry (LC-MS/MS) method has been developed for the measurement of dopamine (DA), 5-hydroxytryptamine (5HT) and norepinephrine (NE) in brain microdialysates. The assay has also been utilised for the simultaneous measurement of these neurotransmitters and cocaine in brain dialysates. The neurotransmitters and cocaine were resolved in a single 4-min run using a binary gradient elution profile. The analytes were detected using tandem mass spectrometry in the positive ion electrospray mode. The limits of detection for DA, NE, 5HT and cocaine were 200, 1000, 900 pM and 1 pg ml(-1), respectively.

Item Type: Article
Research Areas: A. > School of Science and Technology > Natural Sciences
Item ID: 22823
Useful Links:
Depositing User: Ajit Shah
Date Deposited: 03 Nov 2017 11:59
Last Modified: 28 Nov 2019 14:02
URI: https://eprints.mdx.ac.uk/id/eprint/22823

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