Analysis of procainamide-derivatised heparan sulphate disaccharides in biological samples using hydrophilic interaction liquid chromatography mass spectrometry
Antia, Imeobong U., Mathew, Kurian, Yagnik, Darshna, Hills, Frank ORCID: https://orcid.org/0000-0001-8235-7545 and Shah, Ajit J.
ORCID: https://orcid.org/0000-0002-2350-6384
(2018)
Analysis of procainamide-derivatised heparan sulphate disaccharides in biological samples using hydrophilic interaction liquid chromatography mass spectrometry.
Analytical and Bioanalytical Chemistry, 410
(1)
.
pp. 131-143.
ISSN 1618-2642
[Article]
(doi:10.1007/s00216-017-0703-1)
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Abstract
Glycosaminoglycans (GAGs) are a family of linear heteropolysaccharides made up of repeating disaccharide units that are found on the surface and extracellular matrix of animal cells. They are known to play a critical role in a wide range of cellular processes including proliferation, differentiation and invasion. To elucidate the mechanism of action of these molecules, it is essential to quantify their disaccharide composition. Analytical methods that have been reported involve either chemical or enzymatic depolymerisation of GAGs followed by separation of non-derivatised (native) or derivatised disaccharide subunits and detection by either UV/fluorescence or MS. However, the measurement of these disaccharides is challenging due to their hydrophilic and labile nature. Here we report a pre-column LC-MS method for the quantification of GAG disaccharide subunits. Heparan sulphate (HS) was extracted from cell lines using a combination of molecular weight cutoff and anion exchange spin filters and digested using a mixture of heparinases I, II and III. The resulting subunits were derivatised with procainamide, separated using hydrophilic interaction liquid chromatography and detected using electrospray ionisation operated in positive ion mode. Eight HS disaccharides were separated and detected together with an internal standard. The limit of detection was found to be in the range 0.6–4.9 ng/mL. Analysis of HS extracted from all cell lines tested in this study revealed a significant variation in their composition with the most abundant disaccharide being the non-sulphated ∆UA–GlcNAc. Some structural functional relationships are discussed demonstrating the viability of the pre-column method for studying GAG biology
Item Type: | Article |
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Research Areas: | A. > School of Science and Technology > Natural Sciences > Biomarkers for Cancer group |
Item ID: | 22778 |
Notes on copyright: | This is a post-peer-review, pre-copyedit version of an article published in Analytical and Bioanalytical Chemistry. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00216-017-0703-1 |
Useful Links: | |
Depositing User: | Ajit Shah |
Date Deposited: | 01 Nov 2017 10:05 |
Last Modified: | 29 Nov 2022 20:14 |
URI: | https://eprints.mdx.ac.uk/id/eprint/22778 |
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