Rapid identification of E. coli bacteriophages using mass spectrometry
Serafim, Vlad, Ring, Christopher J. 
ORCID: https://orcid.org/0000-0002-9278-0942, Pantoja Munoz, Leonardo, Shah, H. and Shah, Ajit J. ORCID: https://orcid.org/0000-0002-2350-6384
(2017)
Rapid identification of E. coli bacteriophages using mass spectrometry.
Journal of Proteomics and Enzymology, 6
(1)
, 1000130.
pp. 1-5.
ISSN 2470-1289
[Article]
(doi:10.4172/2470-1289.1000130)
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Abstract
Objective: The current increasing interest in the application of mass spectrometry, in particular MALDI-TOF MS, to identification of bacteria and fungi calls for a need to utilise this technology for identification of other infectious agents such as viruses. The aim of the present study was to develop a rapid and reliable mass spectrometry-based proteomic method for identification of Escherichia coli phages.
Methods: The approach was based on rapid in-solution tryptic digestion of suspensions of plaque-purified bacteriophage followed by mass spectral analysis. Matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and liquid chromatography – tandem mass spectrometry (LC-MS) were used to analyse the tryptic digests. Processing of tandem mass spectrometry data and interpretation of results were achieved using Mascot software and the Swiss-Prot database.
Results: Five bacteriophage species (Enterobacteria phages P2, T4, T5, T7 and Lambda) isolated in E. coli cultures were identified. The viral proteins were identified from a complex mixture of host bacterial proteins. In addition, using a single ion monitoring method, a Lambda prophage derived protein was also identified.
Conclusion: The data obtained demonstrate that LC-MS/MS can be used for accurate identification of E.coli- specific bacteriophages in both lytic and lysogenic cycles
Keywords: Bacteriophage virus; Mass-spectrometry; Liquid chromatography; MALDI; LC-MS/MS; Lytic; Lysogenic; Enterobacteria; E.coli; Phage; Viruses
| Item Type: | Article |
|---|---|
| Research Areas: | A. > School of Science and Technology > Natural Sciences |
| Item ID: | 22059 |
| Notes on copyright: | Article is published under open access |
| Useful Links: | |
| Depositing User: | Ajit Shah |
| Date Deposited: | 16 Jun 2017 15:59 |
| Last Modified: | 29 Nov 2022 21:10 |
| URI: | https://eprints.mdx.ac.uk/id/eprint/22059 |
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