Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor
Wang, Scarlet Xiaoyan, Acha, Desiree, Shah, Ajit J. ORCID: https://orcid.org/0000-0002-2350-6384, Hills, Frank
ORCID: https://orcid.org/0000-0001-8235-7545, Roitt, Ivan, Demosthenous, Andreas and Bayford, Richard
ORCID: https://orcid.org/0000-0001-8863-6385
(2017)
Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor.
Biosensors and Bioelectronics, 92
.
pp. 482-488.
ISSN 0956-5663
[Article]
(doi:10.1016/j.bios.2016.10.077)
|
PDF
- Final accepted version (with author's formatting)
Available under License Creative Commons Attribution-NonCommercial-NoDerivatives 4.0. Download (424kB) | Preview |
Abstract
This study presents a novel approach based on a four-electrode electrochemical biosensor for the detection of tau protein – one of the possible markers for the prediction of Alzheimer's disease (AD). The biosensor is based on the formation of stable antibody–antigen complexes on gold microband electrodes covered with a layer of a self-assembled monolayer and protein G. Antibodies were immobilized on the gold electrode surface in an optimal orientation by protein G interaction. Electrochemical impedance spectroscopy was used to analyze impedance change, which revealed a linear response with increasing tau concentrations. The assay is fast (<1 h for incubation and measurement) and very sensitive. The limit of quantification for the full-length 2N4R tau protein is 0.03 pM, a value unaltered when the assay was processed in bovine serum albumin or human serum. This technology could be adapted for the detection of other biomarkers to provide a multiple assay to identify AD progression in a point of care setting.
Item Type: | Article |
---|---|
Research Areas: | A. > School of Science and Technology > Natural Sciences > Biophysics and Bioengineering group |
Item ID: | 20921 |
Notes on copyright: | © 2016. This author's accepted manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
Useful Links: | |
Depositing User: | Richard Bayford |
Date Deposited: | 08 Nov 2016 11:12 |
Last Modified: | 29 Nov 2022 20:50 |
URI: | https://eprints.mdx.ac.uk/id/eprint/20921 |
Actions (login required)
![]() |
View Item |
Statistics
Additional statistics are available via IRStats2.