Agrobacterium rhizogenes rolB gene affects photosynthesis and chlorophyll content in transgenic tomato (Solanum lycopersicum L.) plants

Bettini, Priscilla B., Marvasi, Massimiliano, Fani, Fabiola, Lazzara, Luigi, Cosi, Elena, Melani, Lorenzo and Mauro, Maria Luisa (2016) Agrobacterium rhizogenes rolB gene affects photosynthesis and chlorophyll content in transgenic tomato (Solanum lycopersicum L.) plants. Journal of Plant Physiology, 204 . pp. 27-35. ISSN 0176-1617 [Article] (doi:10.1016/j.jplph.2016.07.010)

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Abstract

Insertion of Agrobacterium rhizogenes rolB gene into plant genome affects plant development, hormone balance and defence. However, beside the current research, the overall transcriptional response and gene expression of rolB as a modulator in plant is unknown. Transformed rolB tomato plant (Solanum lycopersicum L.) cultivar Tondino has been used to investigate the differential expression profile. Tomato is a well-known model organism both at the genetic and molecular level, and one of the most important commercial food crops in the world.

Through the construction and characterization of a cDNA subtracted library, we have investigated the differential gene expression between transgenic clones of rolB and control tomato and have evaluated genes specifically transcribed in transgenic rolB plants. Among the selected genes, five genes encoding for chlorophyll a/b binding protein, carbonic anhydrase, cytochrome b6/f complex Fe-S subunit, potassium efflux antiporter 3, and chloroplast small heat-shock protein, all involved in chloroplast function, were identified. Measurement of photosynthesis efficiency by the level of three different photosynthetic parameters (Fv/Fm, rETR, NPQ) showed rolB significant increase in non-photochemical quenching and a, b chlorophyll content. Our results point to highlight the role of rolB on plant fitness by improving photosynthesis.

Item Type: Article
Additional Information: Available online 25 July 2016
Research Areas: A. > School of Science and Technology
Item ID: 20419
Useful Links:
Depositing User: Massimiliano Marvasi
Date Deposited: 05 Sep 2016 13:32
Last Modified: 01 Jun 2019 17:31
URI: https://eprints.mdx.ac.uk/id/eprint/20419

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