Lentiviral vector transduction of spermatozoa as a tool for the study of early development

Chandrashekran, Anil, Isa, Ihsan, Dudhia, Jayesh, Thrasher, Adrian J., Dibb, Nicholas, Casimir, Colin M. ORCID logoORCID: https://orcid.org/0000-0003-1689-4814, Readhead, Carol and Winston, Robert (2014) Lentiviral vector transduction of spermatozoa as a tool for the study of early development. FEBS Open Bio, 4 (1) . pp. 266-275. ISSN 2211-5463 [Article] (doi:10.1016/j.fob.2014.02.008)

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Spermatozoa and lentiviruses are two of nature’s most efficient gene delivery vehicles. Both can be genetically modified and used independently for the generation of transgenic animals or gene transfer/therapy of inherited disorders. Here we show that mature spermatozoa can be directly transduced with various pseudotyped lentiviral vectors and used in in vitro fertilisation studies. Lentiviral vectors encoding Green Fluorescent Protein (GFP) were shown to be efficiently processed and expressed in sperm. When these transduced sperm were used in in vitro fertilisation studies, GFP expression was observed in arising blastocysts. This simple technique of directly transducing spermatozoa has potential to be a powerful tool for the study of early and pre-implantation development and could be used as a technique in transgenic development and vertical viral transmission studies.

Item Type: Article
Research Areas: A. > School of Science and Technology > Natural Sciences > Molecular Biology group
Item ID: 18363
Notes on copyright: This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/)
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Depositing User: Colin Casimir
Date Deposited: 02 Nov 2015 10:55
Last Modified: 29 Nov 2022 23:47
URI: https://eprints.mdx.ac.uk/id/eprint/18363

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