Comparison of mesenchymal stem cell proliferation and differentiation between biomimetic and electrochemical coatings on different topographic surfaces

García-Gareta, Elena, Hua, Jia ORCID: https://orcid.org/0000-0003-3558-2058, Knowles, Jonathan C. and Blunn, Gordon W. (2013) Comparison of mesenchymal stem cell proliferation and differentiation between biomimetic and electrochemical coatings on different topographic surfaces. Journal of Materials Science: Materials in Medicine, 24 (1) . pp. 199-210. ISSN 0957-4530 [Article] (doi:10.1007/s10856-012-4789-x)

Abstract

The hypothesis for this study was that there is no difference in mesenchymal stem cells (MSCs) proliferation and osteogenic differentiation between calcium-phosphate (CaP) coatings with different crystal size deposited on different topographic surfaces of metal discs. Polished (P) and sand-blasted (SB) tantalum and TiAl6V4 discs were CaP coated by three methods-biomimetic (BioM), electrochemical at 20 mA/cm(2) and at 6.5 mA/cm(2)-and cultured with MSCs. At days 4, 7 and 14, cell proliferation-alamarBlue(®) activity and DNA quantification-and differentiation down the osteogenic lineage-ALP activity normalised per amount of DNA and SEM (morphology)-were analysed. Results showed that MSCs proliferated more when cultured on the nano-sized BioM coatings compared to uncoated and electrochemically coated discs. MSCs also proliferated more on P surfaces than on SB and or electrochemical coatings. All the coatings induced osteogenic differentiation, which was greater on electrochemical coatings and SB discs.

Item Type: Article
Research Areas: A. > School of Science and Technology > Natural Sciences
Item ID: 16431
Useful Links:
Depositing User: Jia Hua
Date Deposited: 28 May 2015 14:03
Last Modified: 30 May 2019 18:32
URI: https://eprints.mdx.ac.uk/id/eprint/16431

Actions (login required)

View Item View Item