Bacillus subtilis fadB (ysiB) gene encodes an enoyl-CoA hydratase

Frandi, Antonio, Zucca, Paolo, Marvasi, Massimiliano, Mastromei, Giorgio, Sanjust, Enrico and Perito, Brunella (2011) Bacillus subtilis fadB (ysiB) gene encodes an enoyl-CoA hydratase. Annals of Microbiology, 61 (2, Jou) . pp. 371-374. ISSN 1590-4261 [Article] (doi:10.1007/s13213-010-0121-5)

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Fatty acids are essential components of membranes and are an important source of metabolic energy. In bacteria, the β-oxidation pathway is well known in Escherichia coli. Bacillus subtilis possesses a considerable number of genes, organized in five operons, that are most likely involved in the β-oxidation of fatty acids. Among these genes, only one product, FadRBs (YsiA), has been recently characterized as a transcriptional regulatory protein which negatively regulates the expression of β-oxidation genes including those belong- ing to the lcfA operon, including fadRBs (ysiA). The probable involvement of the FadRBs (YsiA) regulon members in β- oxidation is inferred from data based on BLASTP similarity of their gene products. In this work, we report the cloning and the expression of B. subtilis fadBBs(ysiB), belonging to the lcfA operon, and the functional characterization of its product as an enoyl-CoA hydratase, demonstrating the actual involvement of these genes in fatty acid β-oxidation.

Item Type: Article
Additional Information: J2: Ann Microbiol
Keywords (uncontrolled): �²-oxidation, Bacillus subtilis, fadB Bs, enoyl-CoA hydratase, lcfA operon
Research Areas: A. > School of Science and Technology > Natural Sciences
Item ID: 15761
Depositing User: Massimiliano Marvasi
Date Deposited: 06 May 2015 10:59
Last Modified: 30 Nov 2022 00:53

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