Rod outer segment membrane guanylate cyclase type 1 (ROS-GC1) calcium-modulated transduction system in the sperm

Jankowska, Anna, Burczynska, Beata ORCID: https://orcid.org/0000-0003-4101-8525, Duda, Teresa and Warchol, Jerzy B. (2010) Rod outer segment membrane guanylate cyclase type 1 (ROS-GC1) calcium-modulated transduction system in the sperm. Fertility and Sterility, 93 (3) . pp. 904-912. ISSN 0015-0282 [Article] (doi:10.1016/j.fertnstert.2008.10.048)

Abstract

OBJECTIVE:
Evaluation of the presence of a Ca(2+)-regulated membrane guanylate cyclase signal transudation system in the spermatozoa.

DESIGN:
Experimental study.

SETTING:
Research university laboratory.

PATIENT(S):
Human sperm obtained from healthy donors who met the criteria of the World Health Organization for normozoospermia and bovine semen collected from bulls of proven fertility.

INTERVENTION(S):
Radioimmunoassay and immunohistochemistry of human and bovine spermatozoa.

MAIN OUTCOME MEASURE(S):
The membrane guanylate cyclase activity and the presence of membrane guanylate cyclase transduction machinery components in the spermatozoa.

RESULT(S):
The identity of a Ca(2+)-modulated membrane guanylate cyclase transduction machinery in human and bovine spermatozoa has been documented. The machinery is both inhibited and stimulated within nanomolar to semimicromolar range of free Ca(2+). The transduction component of this machinery is the rod outer segment membrane guanylate cyclase type 1 (ROS-GC1). The enzyme coexists with three Ca(2+)-dependent modulators: guanylate cyclase activating protein type 1 (GCAP1), S100B and neurocalcin delta. ROS-GC1 and its modulators are present in the heads and tails of both species' spermatozoa.

CONCLUSION(S):
The coexpression of ROS-GC1 and its activators in spermatozoa suggests that the Ca(2+)-modulated ROS-GC1 transduction system may be a part of the fertilization machinery.

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