Ectopic HCG beta may induce epithelialmensenchymal transition on human kera tinocytes in vitro and this could promote tumour progression and invasion.
Wen, Xuesong and Li, Dong and Ghali, Lucy and Iles, Ray K. (2010) Ectopic HCG beta may induce epithelialmensenchymal transition on human kera tinocytes in vitro and this could promote tumour progression and invasion. Tumour Biology, 31 (S44). ISSN 1010-4283
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Aims: Our more recent work on cervical cancer showed that there was a positive correlation between hCGbeta expression and tumour progression, invasion and metastasis. However, the mechanism by which hCGbeta participates in these malignant aspect of oncogenesis is still unknown. Our most recent study has suggested that the hCGbeta expressed by tumors is structurally different from that derived during pregnancy. This study aims to examine the effect of tumour cell derived hCGbeta on cellular transformation. Methods: Conditioned medium (CM) from ScaBER cell line (HTB-3, ATCC), a stable bladder cancer cell line that is known to over express hCGbeta, was used to incubate human keratinocytes (HK, ScienCell) in culture. HK cells were also incubated with either conditioned media from TCL-1 (trophoblast cell line) or 3T3 cells (mouse fibroblast cell line) or by adding recombinant hCGbeta to control medium. HK cells were cultured for 72 hours and cellular morphological changes observed. The expression of Cytokeratin, Vimentin and E-cadherin proteins was studied by immunocytochemistry. The percentage and intensity of positive stained cells were evaluated by averaging of six individual fields under light microscope. Results: Exposure to SCaBER CM resulted in a significant spindled shape change in HK morphology while those treated with TCL-1, 3T3 or recombinant hCGbeta showed little change. Immunohistochemistry staining analysis showed decreased expression of cytokeratin (80% weak to 100%strong), increased expression of Vimentin (75% strong to none staining) and down regulation of E-cadherin (50% medium to 100% strong) in HK that were incubated with SCaBER conditioned media. Conclusion: The data shows that factors present in the SCaBER cell CM transform HK cells from an epithelial to mesenchymal phenotype. Although hCGbeta is secreted in to the culture media the effects maybe due to other factors produced by SCaBER cells.
|Research Areas:||School of Science and Technology > Natural Sciences|
School of Science and Technology > Natural Sciences > Biomarkers for Cancer group
|Deposited On:||27 Jun 2011 10:05|
|Last Modified:||24 Oct 2014 15:39|
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