Immunochemical analysis of the human chorionic gonadotrophin-like material secreted by 'normal' and neoplastic urothelial cells.

Iles, Ray K. and Chard, Tim (1989) Immunochemical analysis of the human chorionic gonadotrophin-like material secreted by 'normal' and neoplastic urothelial cells. Journal of molecular endocrinology., 2 (2). pp. 107-112. ISSN 0952-5041

Full text is not in this repository.

Official URL: http://jme.endocrinology-journals.org/cgi/reprint/...

This item is available in the Library Catalogue

Abstract

Material with the immunochemical characteristics of human chorionic gonadotrophin (hCG) is produced by bladder tumour cells in vitro and in vivo. In order to characterize this material further, media were collected from 17 cell cultures (three choriocarcinomas, seven bladder carcinomas and seven 'normal' urothelium). The hCG-like material was compared with pregnancy hCG and purified alpha-and beta-subunits by specific radioimmunoassays. Media were also submitted to affinity chromatography and the fractions further analysed by SDS-PAGE and Western blotting. It was shown that both the neoplastic and normal urothelium produced only free beta-subunit-like material. This urothelial 'beta-hCG' has the same molecular weight and electrophoretic mobility as that present in the intact hCG of pregnancy.

Item Type:Article
Research Areas:School of Science and Technology > Natural Sciences
ID Code:6114
Deposited On:23 Jun 2010 13:56
Last Modified:11 Oct 2013 05:12

Repository staff and depositor only: item control page

Full text downloads (NB count will be zero if no full text documents are attached to the record)

Downloads per month over the past year