Immunochemical analysis of the human chorionic gonadotrophin-like material secreted by 'normal' and neoplastic urothelial cells.

Iles, Ray K. and Chard, Tim (1989) Immunochemical analysis of the human chorionic gonadotrophin-like material secreted by 'normal' and neoplastic urothelial cells. Journal of molecular endocrinology., 2 (2). pp. 107-112. ISSN 0952-5041

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Abstract

Material with the immunochemical characteristics of human chorionic gonadotrophin (hCG) is produced by bladder tumour cells in vitro and in vivo. In order to characterize this material further, media were collected from 17 cell cultures (three choriocarcinomas, seven bladder carcinomas and seven 'normal' urothelium). The hCG-like material was compared with pregnancy hCG and purified alpha-and beta-subunits by specific radioimmunoassays. Media were also submitted to affinity chromatography and the fractions further analysed by SDS-PAGE and Western blotting. It was shown that both the neoplastic and normal urothelium produced only free beta-subunit-like material. This urothelial 'beta-hCG' has the same molecular weight and electrophoretic mobility as that present in the intact hCG of pregnancy.

Item Type: Article
Research Areas: A. > School of Science and Technology > Natural Sciences
Item ID: 6114
Depositing User: Professor Ray Iles
Date Deposited: 23 Jun 2010 13:56
Last Modified: 16 Dec 2015 16:40
URI: http://eprints.mdx.ac.uk/id/eprint/6114

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