Mechanical strain and estrogen activate estrogen receptor alpha in bone cells.
Jessop, H. L., Sjöberg, M., Cheng, Ming Zhao, Zaman, Gul, Wheeler-Jones, C. P. and Lanyon, Lance E. (2001) Mechanical strain and estrogen activate estrogen receptor alpha in bone cells. Journal of Bone and Mineral Research, 16 (6). pp. 1045-1055. ISSN 0884-0431
Full text is not in this repository.
This item is available in the Library Catalogue
Bone cells' early responses to estrogen and mechanical strain were investigated in the ROS 17/2.8 cell line. Immunoblotting with antiphosphorylated estrogen receptor a (ER-alpha) antibody showed that when these cells were exposed for 10 minutes to estrogen (10(-8) M) or a single period of cyclic dynamic strain (peak 3400 microepsilon, 1 Hz, 600 cycles), there was an increase in the intensity of a 66-kDa band, indicating phosphorylation of ser122 in the amino terminus of ER-alpha. Increased phosphorylation was detected within 5 minutes of exposure to estrogen and 5 minutes after the end of the period of strain. Estrogen and strain also activated the mitogen-activated protein kinase (MAPK) family member extracellular regulated kinase-1 (ERK-1). Increases in ERK activation coincided with increased ER-alpha phosphorylation. Activation of ERK-1 and the phosphorylation of ER-alpha, by both estrogen and strain, were prevented by the MAP kinase kinase (MEK) inhibitor U0126 and the protein kinase A (PKA) inhibitor (PKI). These data support previous suggestions that resident bone cells' early responses to strain and estrogen share a common pathway, which involves ER-alpha. This pathway also appears to involve PKA and ERK-mediated phosphorylation of ser122 within the amino terminus of ER-alpha. Reduced availability of this pathway when estrogen levels are reduced could explain diminished effectiveness of mechanically related control of bone architecture after the menopause.
|Research Areas:||Health & Education > Complementary Health|
|Citations on ISI Web of Science:||58|
|Deposited On:||22 Feb 2010 16:16|
|Last Modified:||15 Oct 2013 09:25|
Repository Staff Only: item control page
Downloads per month over past year