Effect of X and Y box deletions on the development of diabetes in H-2Ea chain transgenic NOD mice.

O'Shea, H. and Yousaf, N. and Altmann, D. and Fehervari, Z. and Tonks, P. and Hetherington, C. and Harach, S. and Bland, C. and Cooke, Anne and Lund, Torben (2006) Effect of X and Y box deletions on the development of diabetes in H-2Ea chain transgenic NOD mice. Scandinavian journal of immunology. , 63 (1). pp. 17-25. ISSN 0300-9475

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Abstract

The development of type 1 diabetes in nonobese diabetic (NOD) mice is influenced by major histocompatibility complex (MHC) class II genes. The NOD-E transgenic mouse, which expresses H2-E as a result of the introduction of an Ead gene, is protected from development of type 1 diabetes. While the mechanism of protection remains unclear, the effect has been regarded as a model system for MHC protection from autoimmunity. We have investigated the effect of deletions of the Ea promoter region, which, in turn, affect H2-E expression patterns in transgenic NOD mice. We have constructed transgenic NOD mice where the X (ΔX) and Y (ΔY) boxes of the Ead gene have, respectively, been functionally deleted. Previous reports, using X- or Y-box-deleted H2-E transgenic mice, made by crossing the appropriate transgenes onto the NOD background from C57BL/6 transgenic mice, indicated that promoter mutation abrogated the H2-E-mediated protection seen in NOD-E. The NOD ΔX and NOD ΔY transgenic mice generated in the present study differ in susceptibility to diabetes from wild-type NOD mice. NOD ΔY1 animals are protected from diabetes development, while ΔX mice remain susceptible, albeit to a lesser extent than the parental NOD strain.

Item Type:Article
Research Areas:Middlesex University Schools and Centres > School of Science and Technology > Natural Sciences
ID Code:3977
Deposited On:04 Feb 2010 15:59
Last Modified:15 Oct 2013 11:52

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