Regulation of human growth hormone receptor gene expression by human growth hormone in a human hepatoma cell line
Mullis, Primus E. and Lund, Torben and Patel, Mukesh S. and Brook, Charles G. D. and Brickell, Paul M. (1991) Regulation of human growth hormone receptor gene expression by human growth hormone in a human hepatoma cell line. Molecular and cellular endocrinology, 76 (1-3). pp. 125-133. ISSN 0303-7207
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We have investigated the effects of recombinant human growth hormone (r-hGH) on the expression of hGH-receptor in a human hepatoma cell line (HuH 7). Levels of hGH-receptor mRNA in HuH 7 cells treated with different doses of r-hGH were measured by means of an RNase protection assay. Treatment with r-hGH at physiological concentrations (12.5, 25 and 50 ng/ml) resulted in an increase in hGH-receptor mRNA levels within 1 h of addition of the hormone. A steady state was reached after 3–4 h and maintained for at least 48 h. In contrast, treatment with supraphysiological r-hGH concentrations (150 and 500 ng/ml) led to a down-regulation of hGH-receptor mRNA levels during the first 3 h after hormone addition followed by an increase in hGH-receptor mRNA levels thereafter. Nuclear run-off assays demonstrated that these changes in hGH-receptor mRNA levels were a result of changes in the rate of transcription of the hGH-receptor gene. Cycloheximide (10 μg/ml) did not affect these changes in hGH-receptor gene transcription significantly, indicating that they are mediated by pre-existing factors and do not require new protein synthesis. These data demonstrate that r-hGH specifically regulates the rate of transcription of the hGH-receptor gene in a human hepatoma cell line.
|Research Areas:||Middlesex University Schools and Centres > School of Science and Technology > Natural Sciences|
|Citations on ISI Web of Science:||36|
|Deposited On:||02 Feb 2010 08:30|
|Last Modified:||22 Oct 2013 07:46|
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