Identification of candidate substrates for ectodomain shedding by the metalloprotease-disintegrin ADAM8.

Naus, Silvia and Reipschläger, Simone and Wildeboer, Dirk and Lichtenthaler, Stefan F. and Mitterreiter, Stefan and Guan, Ziqiang and Moss, Marcia L. and Bartsch, Jorg W. (2006) Identification of candidate substrates for ectodomain shedding by the metalloprotease-disintegrin ADAM8. Biological Chemistry, 387 (3). pp. 337-346. ISSN 1431-6730

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Abstract

ADAM proteases are type I transmembrane proteins with extracellular metalloprotease domains. As for most ADAM family members, ADAM8 (CD156a, MS2) is involved in ectodomain shedding of membrane proteins and is linked to inflammation and neurodegeneration. To identify potential substrates released under these pathologic conditions, we screened 10-mer peptides representing amino acid sequences from extracellular domains of various membrane proteins using the ProteaseSpot™ system. A soluble ADAM8 protease containing a pro- and metalloprotease domain was expressed in E. coli and purified as active protease owing to autocatalytic prodomain removal. From 34 peptides tested in the peptide cleavage assay, significant cleavage by soluble ADAM8 was observed for 14 peptides representing membrane proteins with functions in inflammation and neurodegeneration, among them the β-amyloid precursor protein (APP). The in vivo relevance of the ProteaseSpot™ method was confirmed by cleavage of full-length APP with ADAM8 in human embryonic kidney 293 cells expressing tagged APP. ADAM8 cleaved APP with similar efficiency as ADAM10, whereas the inactive ADAM8 mutant did not. Exchanging amino acids at defined positions in the cleavage sequence of myelin basic protein (MBP) revealed sequence criteria for ADAM8 cleavage. Taken together, the results allowed us to identify novel candidate substrates that could be cleaved by ADAM8 in vivo under pathologic conditions.

Item Type:Article
Keywords (uncontrolled):ADAM protease, candidate substrates, ectodomain shedding, fluorescence assay, peptide cleavage
Research Areas:School of Science and Technology > Natural Sciences
Citations on ISI Web of Science:38
ID Code:3876
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Deposited On:28 Jan 2010 16:10
Last Modified:06 Feb 2013 11:44

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