Preparation and immunogenicity of an inactivated hepatitis A vaccine
Pellegrini, Vittoria and Fineschi, Nicoletta and Matteucci, G. and Marsili, I. and Nencioni, L. and Puddu, M. and Garelick, Hemda and Zuckerman, Arie J. (1993) Preparation and immunogenicity of an inactivated hepatitis A vaccine. Vaccine, 11 (3). pp. 383-387. ISSN 0264-410X
Full text is not in this repository.
Official URL: http://dx.doi.org/10.1016/0264-410X(93)90204-B
This item is available in the Library Catalogue
A hepatitis A vaccine was prepared by formaldehyde inactivation of purified hepatitis A virus (HAV) LSH/S strain grown on human diploid MRC-5 cells. The vaccine was devoid of residual infectivity in vitro and failed to induce in marmoset monkeys any pathological features or variations of haematological and clinical chemistry values. Infectious HAV particles were not detected in faeces and sera of the vaccinated primates by ELISA or after passages in MRC-5 cells. The immunogenicity of the vaccine was evaluated by injecting guinea-pigs with 0.8, 0.2 or 0.05 μg of HAV antigen adsorbed onto 0.5 and 1 mg of Al(OH)3 or 0.3 mg of AlPO4. The antibody response, measured by a competitive radioimmunoassay, was dose- and adjuvant-dependent. One injection of 0.2 μg of AlPO4-adsorbed HAV antigen induced seroconversion in 100% of animals and high levels of specific and neutralizing serum antibodies. A further increase of antibody titres was observed after the second and third inoculations. These results show that this vaccine formulation is safe and immunogenic in animal models, and suggest that it should be evaluated further by human clinical studies.
|Research Areas:||School of Science and Technology > Natural Sciences|
|Citations on ISI Web of Science:||21|
|Deposited On:||18 Jan 2010 07:24|
|Last Modified:||13 May 2014 15:51|
Repository staff and depositor only: item control page
Full text downloads (NB count will be zero if no full text documents are attached to the record)
Downloads per month over the past year