Hepatitis C antibody profile and viraemia prevalence in adults with severe haemophilia

Tedder, Richard S. and Briggs, M. and Ring, Christopher J. and Tuke, Philip W. and Jones, P. and Savidge, G. F. and Rodgers, B. and Garson, Jeremy A. (1991) Hepatitis C antibody profile and viraemia prevalence in adults with severe haemophilia. British Journal of Haematology, 79 (3). pp. 512-515. ISSN 0007-1048

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Official URL: http://dx.doi.org/10.1111/j.1365-2141.1991.tb08064...

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Abstract

Sera from 21 patients who had received large amounts of unheated factor VIII concentrate were tested for antibodies to the hepatitis C virus (HCV) by both commercial (Ortho C100) and 'in house' ELISAs. 'In house' assays utilized recombinant structural (core) or non-structural (replicase) HCV proteins generated by a baculovirus expression system. Antibodies to HCV were detected in 100% of the sera by the core protein based ELISA but in only 62% and 19% by the C100 and replicase based ELISAs, respectively. Hepatitis C viraemia was demonstrated in 90% of the patients by in vitro amplification of the 5' non-coding region of the HCV genome. Amplification with primer sets from two other regions of the genome proved less efficient at detecting viraemia. We conclude that the prevalence of hepatitis C infection in haemophiliacs may have been underestimated previously and that almost all HCV-infected patients have evidence of on-going viral replication.

Item Type:Article
Research Areas:School of Science and Technology > Natural Sciences
ID Code:3330
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Deposited On:03 Dec 2009 14:45
Last Modified:10 Jan 2014 06:17

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