Transactivation of the human papilloma virus 16 octamer motif by the octamer binding protein Oct-2 requires both the N and C terminal activation domains
Morris, Peter J. and Ring, Christopher J. and Lillycrop, Karen A. and Latchman, David S. (1993) Transactivation of the human papilloma virus 16 octamer motif by the octamer binding protein Oct-2 requires both the N and C terminal activation domains. Nucleic acids research, 21 (19). pp. 4506-10. ISSN 0305-1048
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The upstream regulatory region (URR) of the human papillomaviruses HPV16 and 18 contains a sequence with a seven out of eight base match to the consensus binding site for octamer binding transcription factors. This motif acts as a target for repression by the Oct-1 transcription factor and therefore inhibits promoter activity in non-cervical cells expressing only Oct-1. In contrast the HPV octamer motif activates promoter activity in cervical cells. Here we show that cervical cells express the activating form of the Oct-2 transcription factor, Oct 2.1 and that this factor can transactivate promoter activity via the HPV16 octamer. This effect is dependent upon both the N and C-terminal activation domains of Oct-2. The expression of specific octamer binding proteins such as Oct-2 in cervical cells thus allows the HPV16 motif to produce opposite effects on gene expression in cervical and non-cervical cells suggesting that it may play a role in the cervical specificity of URR driven gene expression.
|Research Areas:||A. > School of Science and Technology > Natural Sciences|
|Depositing User:||Dr Christopher Ring|
|Date Deposited:||03 Dec 2009 11:56|
|Last Modified:||24 Mar 2015 13:11|
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