Post-transcriptional regulation of the chicken thymidine kinase gene

Groudine, M. and Casimir, Colin M. (1984) Post-transcriptional regulation of the chicken thymidine kinase gene. Nucleic Acids Research, 12 (3). pp. 1427-46. ISSN 0305-1048

Full text is not in this repository.

Official URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC318587...

This item is available in the Library Catalogue

Abstract

In attempting to understand the molecular basis of the control of chicken thymidine kinase (cTK) gene expression, we have examined the steady state cTK RNA content, and the patterns of DNA methylation, chromatin structure and endogenous nuclear runoff transcription of this gene in dividing and non-dividing cells. Our results reveal that the steady state level of cTK poly A+ RNA is correlated with the divisional activity of normal avian cells and tissues. However, no differences in the pattern of Hpa II site methylation or chromatin structure are found among cells containing high or undetectable levels of steady state cTK RNA. In addition, no differences in cTK transcription as assayed by nuclear runoff experiments are detectable in isolated nuclei derived from dividing or non-dividing cells containing high or low levels of steady state cTK RNA. These results suggest that the principal control of chicken thymidine kinase gene expression is post-transcriptional in nature.

Item Type:Article
Research Areas:Middlesex University Schools and Centres > School of Science and Technology > Natural Sciences
Middlesex University Schools and Centres > School of Science and Technology > Natural Sciences > Molecular Biology group
Citations on ISI Web of Science:106
ID Code:3306
Deposited On:02 Dec 2009 08:56
Last Modified:09 Oct 2014 11:56

Repository staff only: item control page

Full text downloads (NB count will be zero if no full text documents are attached to the record)

Downloads per month over the past year