Structure and expression of a newt cardio-skeletal myosin gene. Implications for the C value paradox
Casimir, Colin M. and Gates, P. B. and Ross-Macdonald, P. B. and Jackson, J. F. and Patient, R. K. and Brockes, J. P. (1988) Structure and expression of a newt cardio-skeletal myosin gene. Implications for the C value paradox. Journal of Molecular Biology, 202 (2). pp. 287-296. ISSN 0022-2836
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As part of our studies on the fate of the muscle lineage during amphibian limb regeneration, we have isolated genomic and cDNA sequences from a myosin heavy chain in the newt (Notophthalmus viridescens). Notwithstanding the technical problems inherent in analysing the large newt genome, genomic and cDNA sequences have been isolated and subjected to analysis by restriction mapping. Northern hybridization, Southern hybridization and DNA sequencing. We believe these to be the first single copy newt gene sequences to have been subjected to this type of analysis. The newt gene sequences showed a striking difference from mammalian myosins in both the estimated sizes of the gene and its intervening sequences; these being much larger than in the mammalian models, it is speculated that this could contribute to the exceptional size of the newt genome. By contrast, the coding sequences displayed very high levels of sequence homology to mammalian myosins. In particular, the amino acid sequence of the newt myosin was found to have greatest homology with rat and human myosin isotypes having a similar cardio-skeletal muscle expression pattern. Despite a long evolutionary separation, newt and mammalian cardio-skeletal myosins have remained more similar to each other than have the human or rat cardiac forms to skeletal myosins within their own respective species.
|Research Areas:||School of Science and Technology > Natural Sciences|
|Citations on ISI Web of Science:||18|
|Deposited On:||02 Dec 2009 08:41|
|Last Modified:||13 Jan 2014 08:32|
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