Targeted retroviral transduction of c-kit+ hematopoietic cells using novel ligand display technology
Chandrashekran, Anil and Gordon, Myrtle Y. and Casimir, Colin M. (2004) Targeted retroviral transduction of c-kit+ hematopoietic cells using novel ligand display technology. Blood, 104 (9). pp. 2697-2703. ISSN 0006-4971
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Gene therapy for a wide variety of disorders would be greatly enhanced by the development of vectors that could be targeted for gene delivery to specific populations of cells. We describe here high-efficiency targeted transduction based on a novel targeting strategy that exploits the ability of retroviruses to incorporate host cell proteins into the surface of the viral particle as they bud through the plasma membrane. Ecotropic retroviral particles produced in cells engineered to express the membrane-bound form of stem cell factor (mbSCF) transduce both human cell lines and primary cells with high efficiency in a strictly c-kit (SCF receptor)-dependent fashion. The availability of efficient targeted vectors provides a platform for the development of a new generation of therapies using in vivo gene delivery.
|Research Areas:||A. > School of Science and Technology > Natural Sciences
A. > School of Science and Technology > Natural Sciences > Molecular Biology group
|Depositing User:||Dr Colin Casimir|
|Date Deposited:||02 Dec 2009 14:46|
|Last Modified:||14 Dec 2015 19:00|
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