Characterisation of the differential expression of marker antigens by normal and malignant endometrial epithelium

Chatzaki, E. and Gallagher, C. J. and Iles, Ray K. and Ind, T. E. and Nouri, A. M. and Bax, C. M. R. and Grudzinskas, J. G. (1994) Characterisation of the differential expression of marker antigens by normal and malignant endometrial epithelium. British Journal of Cancer, 69 (6). pp. 1010-1014. ISSN 0007-0920

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Official URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC196942...

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Abstract

In order to examine the production of marker proteins, a reproducible method has been established for culturing purified epithelial cells from normal and malignant endometrium. We have examined the differential expression of secretory proteins using immunohistochemistry in frozen tissue sections, immunocytochemistry in cell cultures derived from the same specimens and protein assays on the culture supernatants. Placental protein 14 (PP14) was produced by normal premenopausal epithelium but not by the post-menopausal or malignant endometrial epithelium. In contrast, placental alkaline phosphatase (PLAP) was produced by endometrial cancers and the endometrial adenocarcinoma-derived cell line Ishikawa, but not by the normal endometrial epithelium. Other markers such as CA-125, which was produced by both normal and malignant endometrium but not by the cell line, and human chorionic gonadotrophin (beta-hCG), which was produced by Ishikawa cells but not by any of the fresh tissues, were less cancer specific. Placental alkaline phosphatase is a direct product of endometrial cancers that can be readily assayed in serum using this two-site assay to test its clinical usefulness in monitoring patients at risk for endometrial cancer.

Item Type:Article
Research Areas:School of Science and Technology > Natural Sciences
ID Code:2972
Deposited On:02 Nov 2009 15:20
Last Modified:30 Jul 2014 15:59

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