Regulation of human chorionic gonadotropin beta subunit expression in ovarian cancer

Śliwa, Aleksandra ORCID: https://orcid.org/0000-0001-9781-2504, Kubiczak, Marta, Szczerba, Anna ORCID: https://orcid.org/0000-0002-2910-292X, Walkowiak, Grzegorz, Nowak-Markwitz, Ewa, Burczynska, Beata, Butler, Stephen, Iles, Ray, Białas, Piotr ORCID: https://orcid.org/0000-0001-9133-4177 and Jankowska, Anna ORCID: https://orcid.org/0000-0003-4532-2014 (2019) Regulation of human chorionic gonadotropin beta subunit expression in ovarian cancer. BMC cancer, 19 (1). p. 746. ISSN 1471-2407 (doi:10.1186/s12885-019-5960-2)

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Abstract

Expression of human chorionic gonadotropin beta subunit by cancers is extensively documented, yet regulation of the multiple genes that can code for this protein is poorly understood. The aim of the study was to examine the mechanisms regulating CGB gene expression in ovarian cancer. Expression of CGB genes and SP1, SP3, TFAP2A transcription factor genes was evaluated by RT-qPCR. The methylation status of CGB genes promoter regions was examined by methylation-specific PCR. mRNA arising from multiple CGB genes was detected in both ovarian control and malignant tissues. However, expression of CGB3-9 genes was shown to be significantly higher in malignant than healthy ovarian tissues. CGB1 and CGB2 transcripts were shown to be present in 20% of ovarian cancers, but were not detected in any of the control samples. Malignant tissues were characterized by DNA demethylation of CGB promoter regions. In ovarian cancer CGB expression positively correlated with TFAP2A transcripts level and expression of TFAP2A transcription factor was significantly higher in cancer than in control tissues. In contrast SP3 expression level was significantly lower in ovarian tumours than in control ovarian tissue. In ovarian cancers increased expression of human chorionic gonadotropin beta subunit is associated with demethylation of CGB promoter regions. CGB3-9 expression level strongly correlates with expression of the TFAP2A transcription factor. Presence of mRNA arising from CGB1 and CGB2 genes appears to be a unique feature of a subset of ovarian cancers.

Item Type: Article
Additional Information: Article number = 746
Keywords (uncontrolled): AP2 transcription factor, DNA methylation, Gene expression regulation, Human chorionic gonadotropin beta subunit, Ovarian cancer
Research Areas: A. > School of Science and Technology > Natural Sciences > Biomarkers for Cancer group
Item ID: 27356
Notes on copyright: © The Author(s). This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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Depositing User: Jisc Publications Router
Date Deposited: 15 Aug 2019 09:39
Last Modified: 15 Aug 2019 12:23
URI: https://eprints.mdx.ac.uk/id/eprint/27356

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