Effect of glycosaminoglycans on growth factor-stimulated trophoblast invasion

Hills, Frank, Rodd, Zoe and Antia, Imeobong U. (2018) Effect of glycosaminoglycans on growth factor-stimulated trophoblast invasion. In: International Federation of Placenta Associations Conference (IFPA2018), 21-24 Sep 2018, Jikei University School of Medicine in Tokyo, Japan. (doi:10.1016/j.placenta.2018.06.004)

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Abstract

Objectives: To determine the effect of glycosaminoglycans and a series of growth factors on the viability and invasion of the extravillous trophoblast cell line SGHPL4.

Methods: Cells were cultured in Hams F10 media supplemented with fetal bovine serum and L-glutamine. For viability studies cells were seeded into 96-well culture plates (104 cells/well), maintained in serum free medium for 24h and then incubated with glycosaminoglycans (heparin, heparin sulphate and hyaluronic acid; each 100ng/ml) ± growth factors (VEGF, FGFand HB-EGF). Cell viability was measured in cells using the MTS assay. Cellular invasion was assessed using the FluoroBlok invasion assay. Cells were serum-starved for 24 h, incubated with the fluorescent dye DiIC12(3) (10mg/ml) for 1 hour prior to seeding onto an artificial extracellular matrix coated 8 mm FluoroBlok porous membrane inserts (2.5 x 105 cells per insert). Growth factors ± GAGs were added to the cell suspension and the inserts were lowered into a 96-well plate containing 10% fetal calf serum.

Plates were incubated at 37°C for 24h. Invasion was determined by measurement of fluorescence of invaded cells using a fluorescent plate reader (Ex549/Em565 nm).

Results: Cell numbers were significantly increased following incubation with VEGF, FGF and HB-EGF. Cell number was also increased after incubation with each of the glycosaminoglycans tested. The largest increase was observed following incubation with heparin sulphate. Cell numbers were further increased when the GFs were incubated with HS and heparin, but not with hyaluronic acid. Invasion was increased following incubation with VEGF, HBEGF and HGF. Heparan sulphate and heparin increased invasiveness in a dose-dependent manner. In contrast, hyaluronic acid had no significant effect.

Conclusion: This study demonstrates a role for glycosaminoglycans in key features of trophoblast function.

Item Type: Conference or Workshop Item (Poster)
Additional Information: Poster abstract published in Placenta, Volume 69, September 2018, Pages e12-e93
Research Areas: A. > School of Science and Technology > Natural Sciences > Reproductive Biology group
Item ID: 26290
Useful Links:
Depositing User: Frank Hills
Date Deposited: 18 Mar 2019 15:54
Last Modified: 04 Sep 2019 00:08
URI: https://eprints.mdx.ac.uk/id/eprint/26290

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