Interactions between progesterone receptor isoforms in myometrial cells in human labour

Pieber, Doris and Allport, Victoria C. and Hills, Frank and Johnson, Mark R. and Bennett, Phillip R. (2001) Interactions between progesterone receptor isoforms in myometrial cells in human labour. Molecular Human Reproduction, 7 (9). pp. 875-879. ISSN 1460-2407

Full text is not in this repository.

This item is available in the Library Catalogue

Abstract

Progesterone acts to maintain uterine quiescence during pregnancy. In contrast to many other species, no decrease in maternal serum levels of progesterone can be observed in humans before the onset of labour. Therefore, a `functional' progesterone withdrawal in association with labour has been proposed. In humans the progesterone receptor (PR) exists in two isoforms, PR-A and PR-B. While PR-B generally mediates the effects of progesterone upon gene transcription, the role of PR-A during pregnancy, and in parturition, is unknown. In this study, term myometrium cells cultured before the onset of labour were transiently transfected with expression vectors for either PR-A or PR-B. Only those cells expressing PR-B significantly increased expression of a progesterone-sensitive reporter when stimulated with progesterone. Co-transfection of both isoforms of PR demonstrated that PR-A is a dominant repressor of transactivation in these cells. Western blot analysis showed that PR-A is present in human myometrium samples taken only after, but not before, the onset of labour. These data suggest that increased expression of PR-A in human myometrium may contribute to `functional' progesterone withdrawal and the initiation of human labour.

Item Type:Article
Additional Information:

PubMed PMID: 11517295.

Research Areas:School of Science and Technology > Natural Sciences
Citations on ISI Web of Science:85
ID Code:2503
Useful Links:
Deposited On:17 Jun 2009 13:52
Last Modified:03 Feb 2014 08:13

Repository staff and depositor only: item control page

Full text downloads (NB count will be zero if no full text documents are attached to the record)

Downloads per month over the past year