Progesterone represses interleukin-8 and cyclo-oxygenase-2 in human lower segment fibroblast cells and amnion epithelial cells
Loudon, Jenifer A.Z. and Elliott, Catherine L. and Hills, Frank and Bennett, Phillip R. (2003) Progesterone represses interleukin-8 and cyclo-oxygenase-2 in human lower segment fibroblast cells and amnion epithelial cells. Biology of Reproduction, 69 (1). pp. 331-337. ISSN 0006-3363
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Labor is preceded by cervical ripening through upregulation of interleukin (IL)-1β, IL-8, and increased prostaglandin synthesis via inducible type 2 cyclooxygenase (COX-2). Progesterone maintains myometrial quiescence during pregnancy. In this study, we examined the effects of IL-1β and progesterone on IL-8 and prostaglandin E2 (PGE2) synthesis and IL-8 and COX-2 mRNA and promoter activity in amnion cells and lower segment fibroblast (LSF) cells. In both cell types, progesterone had no effect on basal IL-8 or PGE2 synthesis. In LSF cells, IL-1β significantly increased IL-8 and PGE2 synthesis and COX-2 and IL-8 mRNA expression, but progesterone significantly attenuated these effects. In prelabor amnion cells, IL-1β also increased IL-8 and PGE2 synthesis and both COX-2 and IL-8 mRNA and promoter expression; however, progesterone significantly attenuated these effects on IL-8 and PGE2 synthesis and COX-2 expression. In postlabor amnion cells, IL-1β increased IL-8 and PGE2 synthesis and COX-2 expression, but progesterone did not attenuate the effect of IL-1β upon IL-8 synthesis. Progesterone repression of IL-8 and COX-2 in LSF cells suggests that IL-8 and COX-2 have similar regulatory mechanisms in LSF cells and that progesterone may play a role in maintenance of cervical competence. The lack of effect of progesterone on IL-8 in postlabor cells may be the result of downregulation of the progesterone receptor during labor.
PubMed PMID: 12672669.
|Research Areas:||Middlesex University Schools and Centres > School of Science and Technology > Natural Sciences|
Middlesex University Schools and Centres > School of Health and Education > Centre for Investigative and Diagnostic Oncology
Middlesex University Schools and Centres > School of Science and Technology > Natural Sciences > Reproductive Biology group
Middlesex University Schools and Centres > School of Science and Technology > Natural Sciences > Biomarkers for Cancer group
|Citations on ISI Web of Science:||35|
|Deposited On:||17 Jun 2009 13:20|
|Last Modified:||27 Oct 2014 17:29|
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