Competition of As and other Group 15 elements for surface binding sites of an extremophilic Acidomyces acidophilus isolated from a historical tin mining site

Chan, Wai Kit and Wildeboer, Dirk and Garelick, Hemda and Purchase, Diane (2018) Competition of As and other Group 15 elements for surface binding sites of an extremophilic Acidomyces acidophilus isolated from a historical tin mining site. Extremophiles, 22 (5). pp. 795-809. ISSN 1431-0651

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Abstract

An arsenic-resistant fungal strain, designated WKC-1, was isolated from a waste roaster pile in a historical tin mine in Cornwall, UK and successfully identified to be Acidomyces acidophilus using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight tandem mass spectrometry (MALDI-TOF/TOF MS) proteomic-based biotyping approach. WKC-1 showed considerable resistance to As5+ and Sb5+ where the minimal inhibitory concentration (MIC) were 22500 mg L-1 and 100 mg L-1 respectively on Czapex-Dox Agar (CDA) medium; it was substantially more resistant to As5+ than the reference strains CBS 335.97 and CCF 4251. In a modified CDA medium containing 0.02 mg L-1 phosphate, WKC-1 was able to remove 70.30 % of As5+ (100 mg L-1). Sorption experiment showed that the maximum capacity of As5+ uptake was 170.82 mg g-1 dry biomass as predicted by the Langmuir model. The presence of Sb5+ reduced the As5+ uptake by nearly 40%. Based on the Fourier-transform infrared spectroscopy (FT-IR) analysis, we propose that Sb is competing with As for these sorption sites: OH, NH, CH, SO3 and PO4 on the fungal cell surface. To our knowledge, this is the first report on the impact of other Group 15 elements on the biosorption of As5+ in Acidomyces acidophilus.

Item Type: Article
Research Areas: A. > School of Science and Technology > Natural Sciences
Item ID: 24935
Notes on copyright: This is a post-peer-review, pre-copyedit version of an article published in Extremophiles. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00792-018-1039-2
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Depositing User: Diane Purchase
Date Deposited: 12 Sep 2018 17:32
Last Modified: 12 Sep 2018 17:38
URI: http://eprints.mdx.ac.uk/id/eprint/24935

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