Opposing effects of final population density and stress on Escherichia coli mutation rate

Krašovec, Rok and Richards, Huw and Gifford, Danna R. and Belavkin, Roman V. and Channon, Alastair and Aston, Elizabeth and McBain, Andrew J. and Knight, Christopher G. (2018) Opposing effects of final population density and stress on Escherichia coli mutation rate. The ISME journal . ISSN 1751-7362 (Published online first)

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Abstract

Evolution depends on mutations. For an individual genotype, the rate at which mutations arise is known to increase with various stressors (stress-induced mutagenesis-SIM) and decrease at high final population density (density-associated mutation-rate plasticity-DAMP). We hypothesised that these two forms of mutation-rate plasticity would have opposing effects across a nutrient gradient. Here we test this hypothesis, culturing Escherichia coli in increasingly rich media. We distinguish an increase in mutation rate with added nutrients through SIM (dependent on error-prone polymerases Pol IV and Pol V) and an opposing effect of DAMP (dependent on MutT, which removes oxidised G nucleotides). The combination of DAMP and SIM results in a mutation rate minimum at intermediate nutrient levels (which can support 7 × 10  cells ml ). These findings demonstrate a strikingly close and nuanced relationship of ecological factors-stress and population density-with mutation, the fuel of all evolution.

Item Type: Article
Additional Information: ** From PubMed via Jisc Publications Router. ** History: received 26-01-2018; revised 18-05-2018; accepted 20-06-2018.
Research Areas: A. > School of Science and Technology > Computer Science > Artificial Intelligence group
Item ID: 24833
Useful Links:
SWORD Depositor: Jisc Publications Router
Depositing User: Jisc Publications Router
Date Deposited: 29 Aug 2018 10:54
Last Modified: 05 Sep 2018 12:48
URI: http://eprints.mdx.ac.uk/id/eprint/24833

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