Opposing effects of final population density and stress on Escherichia coli mutation rate

Krašovec, Rok, Richards, Huw, Gifford, Danna R., Belavkin, Roman V. ORCID: https://orcid.org/0000-0002-2356-1447, Channon, Alastair, Aston, Elizabeth, McBain, Andrew J. and Knight, Christopher G. (2018) Opposing effects of final population density and stress on Escherichia coli mutation rate. The ISME journal . ISSN 1751-7362 (Published online first) (doi:10.1038/s41396-018-0237-3)

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Evolution depends on mutations. For an individual genotype, the rate at which mutations arise is known to increase with various stressors (stress-induced mutagenesis-SIM) and decrease at high final population density (density-associated mutation-rate plasticity-DAMP). We hypothesised that these two forms of mutation-rate plasticity would have opposing effects across a nutrient gradient. Here we test this hypothesis, culturing Escherichia coli in increasingly rich media. We distinguish an increase in mutation rate with added nutrients through SIM (dependent on error-prone polymerases Pol IV and Pol V) and an opposing effect of DAMP (dependent on MutT, which removes oxidised G nucleotides). The combination of DAMP and SIM results in a mutation rate minimum at intermediate nutrient levels (which can support 7 × 10  cells ml ). These findings demonstrate a strikingly close and nuanced relationship of ecological factors-stress and population density-with mutation, the fuel of all evolution.

Item Type: Article
Additional Information: ** From PubMed via Jisc Publications Router.
** History: received 26-01-2018;
revised 18-05-2018;
accepted 20-06-2018.
Research Areas: A. > School of Science and Technology > Computer Science > Artificial Intelligence group
Item ID: 24833
Useful Links:
Depositing User: Jisc Publications Router
Date Deposited: 29 Aug 2018 10:54
Last Modified: 04 Apr 2019 05:08
URI: https://eprints.mdx.ac.uk/id/eprint/24833

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