The Cytotoxic Effects of Betulin-Conjugated Gold Nanoparticles as Stable Formulations in Normal and Melanoma Cells

Mioc, Marius and Pavel, Ioana Zinuca and Ghiulai, Roxana and Coricovac, Dorina E. and Farcaş, Claudia and Mihali, Ciprian-Valentin and Oprean, Camelia and Serafim, Vlad and Popovici, Ramona A. and Dehelean, Cristina A. and Shtilman, Michael I. and Tsatsakis, Aristidis M. and Şoica, Codruţa (2018) The Cytotoxic Effects of Betulin-Conjugated Gold Nanoparticles as Stable Formulations in Normal and Melanoma Cells. Frontiers in Pharmacology, 9 . p. 429. ISSN 1663-9812

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Abstract

Gold nanoparticles are currently investigated as theranostics tools in cancer therapy due to their proper biocompatibility and increased efficacy related to the ease to customize the surface properties and to conjugate other molecules. Betulin, [lup-20(29)-ene-3β, 28-diol], is a pentacyclic triterpene that has raised scientific interest due to its antiproliferative effect on several cancer types. Herein we described the synthesis of surface modified betulin-conjugated gold nanoparticles using a slightly modified Turkevich method. Transmission electron microscopy (TEM) imaging, dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) were used for the characterization of obtained gold nanoparticles. Cytotoxic activity and apoptosis assessment were carried out using the MTT and Annexin V/PI apoptosis assays. The results showed that betulin coated gold nanoparticles presented a dose-dependent cytotoxic effect and induced apoptosis in all tested cell lines.

Item Type: Article
Additional Information: Article number = 429. ** From PubMed via Jisc Publications Router. ** History: received 12-12-2017; accepted 11-04-2018.
Keywords (uncontrolled): apoptosis, betulin, gold nanoparticles, melanoma, skin
Research Areas: A. > School of Science and Technology > Natural Sciences
Item ID: 24481
Notes on copyright: Copyright © 2018 Mioc, Pavel, Ghiulai, Coricovac, Farcaş, Mihali, Oprean, Serafim, Popovici, Dehelean, Shtilman, Tsatsakis and Şoica. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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SWORD Depositor: Jisc Publications Router
Depositing User: Jisc Publications Router
Date Deposited: 04 Jul 2018 12:10
Last Modified: 08 Sep 2018 17:09
URI: http://eprints.mdx.ac.uk/id/eprint/24481

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