Effective delivery of arsenic trioxide to HPV-positive cervical cancer cells using optimised liposomes: a size and charge study

Akhtar, Anam and Wang, Scarlet Xiaoyan and Ghali, Lucy and Bell, Celia M. and Wen, Xuesong (2018) Effective delivery of arsenic trioxide to HPV-positive cervical cancer cells using optimised liposomes: a size and charge study. International Journal of Molecular Sciences, 19 (4). ISSN 1422-0067

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Abstract

Despite the success of arsenic trioxide (ATO) in treating haematological malignancies, its potential to treat solid tumours has not been fully exploited, owing to its dose-limiting toxicity and poor pharmacokinetics. In order to overcome this hurdle, liposomal encapsulation of the drug with different surface charges (neutral, negative, and positive) and sizes (100, 200 and 400 nm) were synthesised and tested on human papilloma virus (HPV)-positive HeLa and HPV-negative HT-3 cervical cancer cell lines. Two epithelial cell lines-human keratinocytes (HK) and human colon cells (CRL-1790)-were used as controls. The synthesised liposomes were tested for their physico-chemical characteristics, drug loading efficiency, and toxicity on the studied cell lines. Neutral liposomes of 100 nm in size were the chosen formulation for delivering ATO into the studied cells, as they showed the least intrinsic cytotoxicity and the highest loading efficiency. The findings demonstrated that the optimised formulation of liposomes was an effective drug delivery method for HPV-infected cervical cancer cells. Furthermore, the toxicity vs. uptake ratio was highest for HeLa cells, while a reduced or minimal toxic effect was observed for non-HPV-infected cervical cancer cells and control cells. These findings may provide a promising therapeutic strategy for effectively managing cervical cancers.

Item Type: Article
Additional Information: Article number = 1081. ** From PubMed via Jisc Publications Router. ** History: received 20-02-2018; revised 16-03-2018; accepted 03-04-2018.
Keywords (uncontrolled): arsenic trioxide (ATO), cervical cancer, drug delivery, human papilloma virus (HPV), liposome
Research Areas: A. > School of Science and Technology > Natural Sciences
Item ID: 24449
Useful Links:
SWORD Depositor: Jisc Publications Router
Depositing User: Jisc Publications Router
Date Deposited: 03 Jul 2018 13:38
Last Modified: 03 Jul 2018 13:44
URI: http://eprints.mdx.ac.uk/id/eprint/24449

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