An introduction to mass spectrometry based proteomics-detection and characterization of gonadotropins and related molecules
Kicman, Andrew T. and Parkin, Mark C. and Iles, Ray K. (2007) An introduction to mass spectrometry based proteomics-detection and characterization of gonadotropins and related molecules. Molecular and cellular endocrinology, 260-26 . pp. 212-227. ISSN 0303-7207
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This review introduces fundamental aspects of mass spectrometry (MS) based proteomics and illustrates how MS is an effective tool for the analysis of glycoprotein hormones. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) and electrospray ionization (ESI) MS are complementary approaches that have been applied for the analysis of gonadotropins, e.g. to characterize differences in the oligosaccharide distribution of commercial human chorionic gonadotropin preparations, for isolated nicked β-subunit, and identification of a metabolite of placental transforming growth factor in pharmaceutical hCG preparations. Immunoaffinity trapping and concentration of digested sample extract prior to MS analysis confers analytical sensitivity akin to immunoassay. A desirable objective would be to develop for clinical purposes a rapid procedure for MS detection and characterization of gonadotropins. Refinement of on-target immobilization and digestion for subsequent ionization by MALDI may eventually help to provide this capability. The advent of hybrid mass spectrometers will further advance the characterization of these complex molecules.
|Research Areas:||A. > School of Science and Technology > Natural Sciences|
|Citations on ISI Web of Science:||11|
|Deposited On:||20 May 2009 09:09|
|Last Modified:||30 Sep 2015 14:59|
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