High performance liquid chromatography of non-protein amino acids extracted from Acacia seeds

Shah, Ajit J., Younie, David A., Adlard, Max W. and Evans, Christine S. (1992) High performance liquid chromatography of non-protein amino acids extracted from Acacia seeds. Phytochemical Analysis, 3 (1). pp. 20-25. (Published online first)

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Abstract

A sensitive, reversed phase, high performance liquid chromatographic procedure for the analysis of non-protein amino acids has been developed. The method is based on precolumn derivatization with 9-fluoroenylmethyl chloroformate of the non-protein amino acids characteristic of seeds of Acacia species, namely N-acetyldjenkolic acid, diaminopropionic acid and diaminobutyric acid and their acetyl, ureido and oxalyl N-substituted derivatives. The separation of derivatives was achieved by means of a gradient elution programme utilizing two buffers of different pH values and acetonitrile. Following separation, the derivatized amino acids were detected spectrofluorometrically. The levels of non-protein amino acids in seeds of Acacia species, which were representative of three taxonomically distinct groups, were measured by analysis of ethanol extracts.

Item Type: Article
Research Areas: A. > School of Science and Technology > Natural Sciences
Item ID: 22911
Depositing User: Ajit Shah
Date Deposited: 10 Nov 2017 16:29
Last Modified: 10 Nov 2017 16:29
URI: https://eprints.mdx.ac.uk/id/eprint/22911

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