Development of a protocol for the automated analysis of amino acids in brain tissue samples and microdialysates

Shah, Ajit J., de Biasi, Vern, Taylor, Steve G., Roberts, Claire J., Hemmati, Panida, Munton, Richard P., West, Andy, Routledge, Carol and Camilleri, Patrick (1999) Development of a protocol for the automated analysis of amino acids in brain tissue samples and microdialysates. Journal of Chromatography B, 735 (2). pp. 133-140. ISSN 1570-0232

Full text is not in this repository.

Abstract

An automated precolumn derivatisation method has been developed for the measurement of fourteen amino acids in brain tissue and microdialysate samples. The method involves labelling amino acids with naphthalene-2,3-dicarboxaldehyde (NDA) in the presence of cyanide (CN-). The resulting highly stable N-substituted 1-cyanobenz[f]isoindole (CBI) derivatives were separated using a binary gradient elution profile and detected fluorometrically. The order of elution of the derivatised amino acids was confirmed by using liquid chromatography with fluorescence and mass spectrometric detection in tandem. Linear calibration plots were obtained for all amino acids in the range studied (0.2-12.5 microM). The limit of detection for CBI derivatives of amino acids was in the range 5-20 fmol (S/N=2) using a 5 microl injection volume. The method has been used for the measurement of amino acids in microdialysates from rat brain and tissue homogenates from different regions of mouse brain.

Item Type: Article
Research Areas: A. > School of Science and Technology > Natural Sciences
Item ID: 22831
Depositing User: Ajit Shah
Date Deposited: 03 Nov 2017 12:04
Last Modified: 03 Nov 2017 12:06
URI: https://eprints.mdx.ac.uk/id/eprint/22831

Actions (login required)

Edit Item Edit Item