High-performance liquid chromatography/tandem mass spectrometry assay for the rapid high sensitivity measurement of basal acetylcholine from microdialysates

Hows, Mark E. and Organ, Andy J. and Murray, Sarah and Dawson, Lee A. and Foxton, Richard R. and Heidbreder, Christian A. and Hughes, Zoe A. and Lacroix, Laurent L. and Shah, Ajit J. (2002) High-performance liquid chromatography/tandem mass spectrometry assay for the rapid high sensitivity measurement of basal acetylcholine from microdialysates. Journal of Neuroscience Methods, 121 (1). pp. 33-39. ISSN 0165-0270

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Abstract

A high-throughput liquid chromatography tandem mass spectrometry (LC/MS/MS) method has been developed for the analysis of acetylcholine (ACh) in brain dialysates. This separation of ACh is based on cation exchange chromatography with elution buffer consisting of a mixture of ammonium acetate, ammonium formate and acetonitrile. Using isocratic separation conditions, ACh was resolved within a minute and detected using tandem mass spectrometry in the positive ion electrospray mode. The limit of detection for ACh was found to be 1 fmol on column with a S/N ratio of 3:1. The assay has been used routinely for the measurement of ACh in brain dialysates from awake freely moving rats. Furthermore, separation conditions were modified to allow simultaneous measurement of ACh and the acetylcholine esterase inhibitor, neostigmine.

Item Type: Article
Research Areas: A. > School of Science and Technology > Natural Sciences
Item ID: 22808
Depositing User: Ajit Shah
Date Deposited: 02 Nov 2017 16:32
Last Modified: 02 Nov 2017 16:32
URI: http://eprints.mdx.ac.uk/id/eprint/22808

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