Development and application of a sensitive high performance ion-exchange chromatography method for the simultaneous measurement of dopamine, 5-hydroxytryptamine and norepinephrine in microdialysates from the rat brain

Heidbreder, Christian A., Lacroix, Laurent, Atkins, Alan R., Organ, Andrew J., Murray, Sarah, West, Andy and Shah, Ajit J. (2001) Development and application of a sensitive high performance ion-exchange chromatography method for the simultaneous measurement of dopamine, 5-hydroxytryptamine and norepinephrine in microdialysates from the rat brain. Journal of Neuroscience Methods, 112 (2). pp. 135-144. ISSN 0165-0270 (doi:10.1016/S0165-0270(01)00460-5)

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Abstract

A high performance liquid chromatography (HPLC) method based on cation exchange separation has been developed for the measurement of dopamine (DA), 5-hydroxytryptamine (5-HT) and norepinephrine (NE) in microdialysates. The separation conditions have been optimised for using electrochemical detection. All three bioamines were resolved in less than 22 min using isocratic conditions. The optimum oxidation potential for the three bioamines was found to be +0.4 V vs. in situ Ag/AgCl reference electrode. Linear regression analysis of HPLC-peak area as a function of concentrations in the range 1-50 ng x ml(-1) gave coefficients of correlation between 0.998 and 0.999. The limit of detection for DA, 5-HT and NE was found to be between 50 and 100 pg x ml(-1) with a signal to noise ratio of 3:1. The method has been applied to the simultaneous measurement of the three monoamines in microdialysates from the medial prefrontal cortex under basal conditions and following the administration of the antipsychotic drug clozapine (10 mg x kg(-1) s.c.)

Item Type: Article
Research Areas: A. > School of Science and Technology > Natural Sciences
Item ID: 22788
Useful Links:
Depositing User: Ajit Shah
Date Deposited: 01 Nov 2017 10:12
Last Modified: 11 Jun 2019 16:19
URI: https://eprints.mdx.ac.uk/id/eprint/22788

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