Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor

Wang, Scarlet Xiaoyan and Acha, Desiree and Shah, Ajit J. and Hills, Frank and Roitt, Ivan and Demosthenous, Andreas and Bayford, Richard (2017) Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor. Biosensors and Bioelectronics, 92 . pp. 482-488. ISSN 0956-5663

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Abstract

This study presents a novel approach based on a four-electrode electrochemical biosensor for the detection of tau protein – one of the possible markers for the prediction of Alzheimer's disease (AD). The biosensor is based on the formation of stable antibody–antigen complexes on gold microband electrodes covered with a layer of a self-assembled monolayer and protein G. Antibodies were immobilized on the gold electrode surface in an optimal orientation by protein G interaction. Electrochemical impedance spectroscopy was used to analyze impedance change, which revealed a linear response with increasing tau concentrations. The assay is fast (<1 h for incubation and measurement) and very sensitive. The limit of quantification for the full-length 2N4R tau protein is 0.03 pM, a value unaltered when the assay was processed in bovine serum albumin or human serum. This technology could be adapted for the detection of other biomarkers to provide a multiple assay to identify AD progression in a point of care setting.

Item Type: Article
Research Areas: A. > School of Science and Technology > Natural Sciences > Biophysics and Bioengineering group
Item ID: 20921
Useful Links:
Depositing User: Richard Bayford
Date Deposited: 08 Nov 2016 11:12
Last Modified: 06 Sep 2018 07:31
URI: http://eprints.mdx.ac.uk/id/eprint/20921

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