The mechanisms of arsenic bioremediation from water by the green microalgae Chlorella vulgaris

Pantoja Munoz, Leonardo, Purchase, Diane ORCID: https://orcid.org/0000-0001-8071-4385, Jones, Huw, Feldmann, Jörg and Garelick, Hemda ORCID: https://orcid.org/0000-0003-4568-2300 (2014) The mechanisms of arsenic bioremediation from water by the green microalgae Chlorella vulgaris. In: THE BNASS / TraceSpec Tandem Conference, 31 Aug - 04 Sep 2014, Aberdeen, Scotland, UK.

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Abstract

The presence of arsenic (As) in drinking water is a major global public health issue. Chlorella vulgaris (C.vulgaris) is a common green alga that tolerates high levels of As. Focused sonication was used to extract previously unidentified As-GS/PC complexes from C. vulgaris and their integrity was confirmed by HPLC online with simultaneous HR-ICP-MS and ES-MS/MS detection. The response of C. vulgaris when challenged with As(III), As(V) and dimethylarsinic acid (DMA) was assessed through experiments on toxicity, adsorption, efflux, speciation of arsenic (reduction, oxidation and chelation with GSH/PC) and compartmentalisation (flow cytometry). C. vulgaris cells did not produce any As-GS/PC complexes when exposed to As(V) which may indicate that a reduction step is needed for As(V) complexation with GSH/PC. Cells formed DMASV-GS upon exposure to DMA, but this is not part of a detoxification mechanism. It was found that As(III) triggers the formation of arsenic complexes with PC and homophytochelatins (hPC) and their compartmentalisation in vacuoles. The potential of C. vulgaris to bio-remediate arsenic from water is highly selective for the more toxic As(III) (for human life) without the potential hazard to reduce As(V) to As(III).

Item Type: Conference or Workshop Item (Speech)
Research Areas: A. > School of Science and Technology > Natural Sciences
Item ID: 16732
Notes on copyright: Oral presentation, permission not required
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Depositing User: Leonardo Pantoja Munoz
Date Deposited: 08 Jun 2015 11:27
Last Modified: 12 Oct 2019 01:29
URI: https://eprints.mdx.ac.uk/id/eprint/16732

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