Comparison of mesenchymal stem cell proliferation and differentiation between biomimetic and electrochemical coatings on different topographic surfaces

García-Gareta, Elena and Hua, Jia and Knowles, Jonathan C. and Blunn, Gordon W. (2013) Comparison of mesenchymal stem cell proliferation and differentiation between biomimetic and electrochemical coatings on different topographic surfaces. Journal of Materials Science: Materials in Medicine, 24 (1). pp. 199-210. ISSN 0957-4530

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Abstract

The hypothesis for this study was that there is no difference in mesenchymal stem cells (MSCs) proliferation and osteogenic differentiation between calcium-phosphate (CaP) coatings with different crystal size deposited on different topographic surfaces of metal discs. Polished (P) and sand-blasted (SB) tantalum and TiAl6V4 discs were CaP coated by three methods-biomimetic (BioM), electrochemical at 20 mA/cm(2) and at 6.5 mA/cm(2)-and cultured with MSCs. At days 4, 7 and 14, cell proliferation-alamarBlue(®) activity and DNA quantification-and differentiation down the osteogenic lineage-ALP activity normalised per amount of DNA and SEM (morphology)-were analysed. Results showed that MSCs proliferated more when cultured on the nano-sized BioM coatings compared to uncoated and electrochemically coated discs. MSCs also proliferated more on P surfaces than on SB and or electrochemical coatings. All the coatings induced osteogenic differentiation, which was greater on electrochemical coatings and SB discs.

Item Type: Article
Research Areas: A. > School of Science and Technology > Natural Sciences
Item ID: 16431
Notes on copyright: Access to full text restricted pending copyright check.
Depositing User: Jia Hua
Date Deposited: 28 May 2015 14:03
Last Modified: 13 Oct 2016 14:35
URI: http://eprints.mdx.ac.uk/id/eprint/16431

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