Mesenchymal stem cells with increased stromal cell-derived factor 1 expression enhanced fracture healing

Ho, Chih-Yuan, Sanghani, Anita, Hua, Jia, Coathup, Melanie, Kalia, Priya and Blunn, Gordon W. (2015) Mesenchymal stem cells with increased stromal cell-derived factor 1 expression enhanced fracture healing. Tissue Engineering Part A, 21 (3-4) . pp. 594-602. ISSN 1937-3368 (doi:10.1089/ten.tea.2013.0762)

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Abstract

Treatment of critical size bone defects pose a challenge in orthopedics. Stem cell therapy together with cytokines has the potential to improve bone repair as they cause the migration and homing of stem cells to the defect site. However, the engraftment, participation, and recruitment of other cells within the regenerating tissue are important. To enhance stem cell involvement, this study investigated overexpression of stem cells with stromal cell-derived factor 1 (SDF-1) using an adenovirus. We hypothesized that these engineered cells would effectively increase the migration of native cells to the site of fracture, enhancing bone repair. Before implantation, we showed that SDF-1 secreted by transfected cells increased the migration of nontransfected cells. In a rat defect bone model, bone marrow mesenchymal stem cells overexpressing SDF-1 showed significantly (p=0.003) more new bone formation within the gap and less bone mineral loss at the area adjacent to the defect site during the early bone healing stage. In conclusion, SDF-1 was shown to play an important role in accelerating fracture repair and contributing to bone repair in rat models, by recruiting more host stem cells to the defect site and encouraging osteogenic differentiation and production of bone.

Item Type: Article
Research Areas: A. > School of Science and Technology > Natural Sciences
Item ID: 16410
Notes on copyright: This work is licensed under a Creative Commons Attribution 3.0 United States License. You are free to copy, distribute, transmit and adapt this work, but you must attribute this work as ‘‘Tissue Engineering, Part A. Copyright 2014 Mary Ann Liebert, Inc. http://liebertpub.com/tea, used under a Creative Commons Attribution License: http://creativecommons.org/licenses/by/3.0/us/’’
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Depositing User: Jia Hua
Date Deposited: 28 May 2015 11:43
Last Modified: 31 May 2019 02:21
URI: https://eprints.mdx.ac.uk/id/eprint/16410

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