Stable knockdown of hCGβ mRNA expression in bladder cancer cells results in significant inhibition of growth

Burczynska, Beata and Booth, Matthew J. and Iles, Ray K. and Shah, Ankita and Shiled, Ashref and Butler, Stephen A. (2013) Stable knockdown of hCGβ mRNA expression in bladder cancer cells results in significant inhibition of growth. Anticancer Research, 33 (9). pp. 3611-3614. ISSN 1791-7530

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Abstract

Background: Expression of human chorionic gonadotropin beta subunit (hCGβ) by epithelial carcinomas is associated with a poor prognosis and has a proposed autocrine growth effect on cancer cells by inhibition of apoptosis. Material and Methods: We transduced the hCGβ-expressing bladder cancer cell line SCaBER with short hairpin (sh) RNA lentiviral gene-specific (CGB) constructs and determined its impact on the synthesis of hCGβ and the resultant effect on cancer cell growth. Results: Stable CGB gene-silenced clones exhibited a 60%-80% reduction in the level of hCGβ expressed and a reduced growth rate of more than 40% compared to wild-type SCaBER cells. Conclusions: shRNA Lentiviral particles achieve stable knockdown of hCGβ translation in the bladder cancer cell line SCaBER. This transforms the phenotype by reducing hCGβ expression and cell growth rate. This is consistent with the proposed autocrine/paracrine function of ectopic hCGβ expression during oncogenesis.

Item Type: Article
Research Areas: A. > School of Science and Technology > Natural Sciences > Biomarkers for Cancer group
Item ID: 11484
Useful Links:
Depositing User: Devika Mohan
Date Deposited: 18 Sep 2013 08:35
Last Modified: 07 Dec 2018 08:36
URI: http://eprints.mdx.ac.uk/id/eprint/11484

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