The influence of ligand organization on the rate of uptake of gold nanoparticles by colorectal cancer cells

Lund, Torben, Callaghan, Martina, Williams, Phil, Turmaine, Mark, Bachmann, Christof, Rademacher, Tom, Roitt, Ivan and Bayford, Richard (2011) The influence of ligand organization on the rate of uptake of gold nanoparticles by colorectal cancer cells. Biomaterials, 32 (36). pp. 9776-9784. ISSN 0142-9612

Full text is not in this repository.

Abstract

We have explored the uptake of different hydrophilic mono- and dual-ligand gold nanoparticles in colorectal cancer cells in vitro and find that the rate of uptake is dependent on the structural organization of the ligands on the surface of the particles rather than their charge or chemical properties. Gold nanoparticles with 50%PEG-NH2/50% glucose are taken up eighteen fold faster than nanoparticles carrying only PEG-NH2 or glucose. Glutathione-coated gold particles are by far the most efficiently internalized; however, glucose-glutathione dual-ligand nanoparticles are taken up at a thirty fold reduced rate. We found furthermore that the rates are influenced by the cell density and concentration of glucose in the growth medium. Rather than being internalized through a conventional receptor-mediated mechanism the particles appear to be taken up by the cells via an energy-independent diffusion across the cell membrane through pre-existing pores or openings in the lipid bi-layer created by ligands on the gold nanoparticles.

Item Type: Article
Keywords (uncontrolled): Cell culture; gold nanoparticles; colon cancer cells; thiol bonds
Research Areas: A. > School of Science and Technology > Natural Sciences
A. > School of Science and Technology > Natural Sciences > Biomarkers for Cancer group
A. > School of Science and Technology > Natural Sciences > Biophysics and Bioengineering group
Item ID: 11257
Useful Links:
Depositing User: Devika Mohan
Date Deposited: 11 Jul 2013 07:24
Last Modified: 13 Oct 2016 14:27
URI: https://eprints.mdx.ac.uk/id/eprint/11257

Actions (login required)

Edit Item Edit Item