Development and application of a liquid chromatography/tandem mass spectrometric assay for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate in brain slice superfusates and tissue extracts

Shah, Ajit J., De la Fleur, Raul, Atkins, Alan, Slone-Murphy, Julia and Dawson, Lee A. (2008) Development and application of a liquid chromatography/tandem mass spectrometric assay for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate in brain slice superfusates and tissue extracts. Journal of Chromatography B, 876 (2). pp. 153-158. ISSN 1570-0232

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Abstract

A liquid chromatography-tandem mass spectrometric method has been developed for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate. The analytes were separated within 5 min using an anion exchange/reverse phase column. The lower limit of quantification for Glu, NAA and NAAG was found to be 5, 50 and 6 nM, respectively, with a signal-to-noise ratio of 5:1. Using this methodology the basal levels of Glu, NAA and NAAG could be measured consistently in in vitro superfusion samples from rat hippocampus. The assay was also used for measurement of the distribution of Glu, NAA and NAAG in different regions of the rat brain.

Item Type: Article
Keywords (uncontrolled): N-Acetylaspartic acid; N-Acetylaspartylglutamic acid; glutamic acid; liquid chromatography–tandem mass spectrometry; superfusion; Ex vivo
Research Areas: A. > School of Science and Technology > Natural Sciences
A. > School of Science and Technology > Natural Sciences > Reproductive Biology group
A. > School of Science and Technology > Natural Sciences > Biomarkers for Cancer group
Item ID: 11163
Useful Links:
Depositing User: Devika Mohan
Date Deposited: 04 Jul 2013 06:12
Last Modified: 13 Oct 2016 14:27
URI: https://eprints.mdx.ac.uk/id/eprint/11163

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